Complement Factor H antibody (Internal Region)

Catalog No. ABIN374588

The Goat Polyclonal anti-Complement Factor H antibody has been validated for WB. It is suitable to detect Complement Factor H in samples from Human.

Quick Overview for Complement Factor H antibody (Internal Region) (ABIN374588)

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Complement Factor H Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognize isoform a of Complement factor H (CFH) only.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-HLVPDRKKDQYK, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Background: The complement Factor H protein is secreted into the bloodstream and acts in the regulation of complement activation. Mutations leading to changes in this protein have been linked with HUS (hemolytic-uremic syndrome) and chronic hypocomplementemic nephropathy. Factor H is mainly synthesised in the liver but also in macrophages and endothelium. It is primarily aplasma glycoprotein but is also found in platelets and there is a membrane bound form on some leukocytes. Consisting of a single polypeptide, the major form of Factor H has a molecular weight of 155 kDa. There are two truncated forms, a non-glycosylated 49 kDa form and a glycosylated 39-43 kDaform. Plasma concentrations are in the range 200-600 mg/L for the 155 kDa form and 1-5 mg/L for thetruncated forms. Factor H is a major regulatory protein of the complement system. By binding to C3b it either displacesor prevents the binding of Bb (activated Factor B). When bound to Factor H, C3b is susceptible tocleavage by Factor 1 to yield iC3b. Factor H is released or modified following this cleavage. The regulatory role of Factor H is essential because C3bBb is not only a C5 convertase but a C3 convertaseand so has a positive feedback effect, potentially consuming the entire C3 pool if unregulated.Synonyms: CFH, H factor 1, HF, HF1, HF2

Gene ID: 3075

NCBI Accession: NP_000177

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin