ABCB5 antibody (Internal Region)

Catalog No. ABIN374907

Product Details anti-ABCB5 Antibody

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCB5 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Sequence: C-QTQHRNTSKK AQ

Specificity: This antibody is expected to recognize both reported isoforms of Human ABCB5 (NP_848654.3 and NP_001157413.1)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_848654.3 and NP_001157413.1. Genename: ABCB5

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: ABCB5 (ABCB5 Products)

Background: ABCB5 is a plasma membrane transporter able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. ABCB5 is also responsible for the resistance to doxorubicin of a subset of malignant melanomas. ABCB5 has more recently been identified as a marker for human malignant-melanoma-initiating cells.Synonyms: ATP-binding cassette sub-family B member 5

Molecular Weight: 89.8kDa according to NP_848654.3.

Gene ID: 340273

NCBI Accession: NP_001157413

UniProt: Q2M3G0