Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

Catalog No. ABIN376540

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed (ABIN376540)

Target: IgG

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: PE

Application: ELISA, Flow Cytometry (FACS)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Specificity: Reacts with the heavy and light chains of rabbit IgG and the light chains of rabbit IgM

Characteristics: Goat F(ab')2 Anti-Rabbit IgG(H+L), Mouse/Human ads-PE

Purification:

Preadsorption: Mouse/Human adsorbed

Application Details

Application Notes:

• Applications: Quality tested applications include - ELISA , FLISA FC ,
• Other referenced applications include - IHC-FS , IHC-PS , ICC , WB
• Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L

Restrictions: For Research Use only

Handling

Buffer: 0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze!
Protect conjugated products from light.
Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: Store at 2-8°C

References

Nogami, Okada, Ishida, Akiyama, Umezawa, Miura: "Inhibition of the STAT5/Pim Kinase Axis Enhances Cytotoxic Effects of Proteasome Inhibitors on FLT3-ITD-Positive AML Cells by Cooperatively Inhibiting the mTORC1/4EBP1/S6K/Mcl-1 Pathway." in: Translational oncology, Vol. 12, Issue 2, pp. 336-349, (2019) (PubMed).

Nogami, Oshikawa, Okada, Fukutake, Umezawa, Nagao, Kurosu, Miura: "FLT3-ITD confers resistance to the PI3K/Akt pathway inhibitors by protecting the mTOR/4EBP1/Mcl-1 pathway through STAT5 activation in acute myeloid leukemia." in: Oncotarget, Vol. 6, Issue 11, pp. 9189-205, (2016) (PubMed).

Target Details

Target: IgG

Target Type: Antibody