GFP Tag antibody

Catalog No. ABIN387748

This Mouse Monoclonal antibody specifically detects GFP Tag in WB and IF. It exhibits reactivity toward Human and has been mentioned in 23+ publications.

Quick Overview for GFP Tag antibody (ABIN387748)

Target: GFP Tag

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP Tag antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 168AT1211

Product Details anti-GFP Tag Antibody

Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged GFP protein was used to produced this monoclonal antibody.

Isotype: IgG1

Application Details

Application Notes: IF: 1:25. WB: 1:20000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified monoclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.

Expiry Date: 6 months

References for anti-GFP Tag antibody (ABIN387748)

Karam, Blanchet, Vivès, Boisguérin, Boudehen, Kremer, Daher: "Mycobacterium abscessus alkyl hydroperoxide reductase C promotes cell invasion by binding to tetraspanin CD81." in: iScience, Vol. 26, Issue 2, pp. 106042, (2023) (PubMed).

Cammareri, Rose, Vincent, Wang, Nagano, Libertini, Ridgway, Athineos, Coates, McHugh, Pourreyron, Dayal, Larsson, Weidlich, Spender, Sapkota, Purdie, Proby, Harwood, Leigh, Clevers, Barker, Karlsson et al.: "Inactivation of TGFβ receptors in stem cells drives cutaneous squamous cell carcinoma. ..." in: Nature communications, Vol. 7, pp. 12493, (2018) (PubMed).

Arumughan, Roske, Barth, Forero, Bravo-Rodriguez, Redel, Kostova, McShane, Opitz, Faelber, Rau, Mielke, Daumke, Selbach, Sanchez-Garcia, Rocks, Panáková, Heinemann, Wanker: "Quantitative interaction mapping reveals an extended UBX domain in ASPL that disrupts functional p97 hexamers." in: Nature communications, Vol. 7, pp. 13047, (2018) (PubMed).

Colinot, Garbuz, Bosland, Wang, Rice, Sullivan, Arrizabalaga, Jerde: "The common parasite Toxoplasma gondii induces prostatic inflammation and microglandular hyperplasia in a mouse model." in: The Prostate, Vol. 77, Issue 10, pp. 1066-1075, (2017) (PubMed).

Zhao, Hu, Zhang, Jiang, Ma, Li, Peng, Chen: "Dual Roles of Two Isoforms of Autophagy-related Gene ATG10 in HCV-Subgenomic replicon Mediated Autophagy Flux and Innate Immunity." in: Scientific reports, Vol. 7, Issue 1, pp. 11250, (2017) (PubMed).

Ghosh, Geahlen: "Stress Granules Modulate SYK to Cause Microglial Cell Dysfunction in Alzheimer's Disease." in: EBioMedicine, Vol. 2, Issue 11, pp. 1785-98, (2016) (PubMed).

Liu, Yang, Suzuki, Tsukita, Ishii, Zhou, Wang, Cao, Qian, Taylor, Oh, Levitan, Ye, Carnegie, Zhao, Malik, Xu: "Moesin and myosin phosphatase confine neutrophil orientation in a chemotactic gradient." in: The Journal of experimental medicine, Vol. 212, Issue 2, pp. 267-80, (2015) (PubMed).

Cohen, Erb, Pogliano, Golden: "Best practices for fluorescence microscopy of the cyanobacterial circadian clock." in: Methods in enzymology, Vol. 551, pp. 211-21, (2015) (PubMed).

Rooj, Liu, McNicholas, Fuller: "Physical and Functional Interactions between a Glioma Cation Channel and Integrin ?1 Require ?-Actinin." in: American journal of physiology. Cell physiology, pp. ajpcell.00036.2015, (2015) (PubMed).

Reddy, Dai, McNicholas, Fuller, Kappes, DeLucas: "Expression and purification of the alpha subunit of the epithelial sodium channel, ENaC." in: Protein expression and purification, (2015) (PubMed).

Passaro, Volpe, Botta, Scamardella, Perruolo, Gillespie, Libertini, Portella: "PARP inhibitor olaparib increases the oncolytic activity of dl922-947 in in vitro and in vivo model of anaplastic thyroid carcinoma." in: Molecular oncology, (2014) (PubMed).

Kearse, Ireland, Prem, Chen, Ware: "RpL22e, but not RpL22e-like-PA, is SUMOylated and localizes to the nucleoplasm of Drosophila meiotic spermatocytes." in: Nucleus (Austin, Tex.), Vol. 4, Issue 3, pp. 241-58, (2013) (PubMed).

Chen, Gubbels: "The Toxoplasma gondii centrosome is the platform for internal daughter budding as revealed by a Nek1 kinase mutant." in: Journal of cell science, Vol. 126, Issue Pt 15, pp. 3344-55, (2013) (PubMed).

Rooj, McNicholas, Bartoszewski, Bebok, Benos, Fuller: "Glioma-specific cation conductance regulates migration and cell cycle progression." in: The Journal of biological chemistry, Vol. 287, Issue 6, pp. 4053-65, (2012) (PubMed).

Qadri, Cormet-Boyaka, Rooj, Lee, Parpura, Fuller, Berdiev: "Low temperature and chemical rescue affect molecular proximity of DeltaF508-cystic fibrosis transmembrane conductance regulator (CFTR) and epithelial sodium channel (ENaC)." in: The Journal of biological chemistry, Vol. 287, Issue 20, pp. 16781-90, (2012) (PubMed).

Ramos, Guy, Chen, Proenca, Gardenghi, Casu, Follenzi, Van Rooijen, Grady, de Sousa, Rivella: "Enhanced erythropoiesis in Hfe-KO mice indicates a role for Hfe in the modulation of erythroid iron homeostasis." in: Blood, Vol. 117, Issue 4, pp. 1379-89, (2011) (PubMed).

Kapoor, Lee, Clark, Bartoszewski, McNicholas, Latham, Bebok, Parpura, Fuller, Palmer, Benos: "Interaction of ASIC1 and ENaC subunits in human glioma cells and rat astrocytes." in: American journal of physiology. Cell physiology, Vol. 300, Issue 6, pp. C1246-59, (2011) (PubMed).

Maier, Ogihara, Trace, Tersey, Robbins, Chakrabarti, Nunemaker, Stull, Taylor, Thompson, Dondero, Lewis, Dinarello, Nadler, Mirmira: "The unique hypusine modification of eIF5A promotes islet beta cell inflammation and dysfunction in mice." in: The Journal of clinical investigation, Vol. 120, Issue 6, pp. 2156-70, (2010) (PubMed).

Lorestani, Sheiner, Yang, Robertson, Sahoo, Brooks, Ferguson, Striepen, Gubbels: "A Toxoplasma MORN1 null mutant undergoes repeated divisions but is defective in basal assembly, apicoplast division and cytokinesis." in: PLoS ONE, Vol. 5, Issue 8, pp. e12302, (2010) (PubMed).

Clark, Jovov, Rooj, Fuller, Benos: "Proteolytic cleavage of human acid-sensing ion channel 1 by the serine protease matriptase." in: The Journal of biological chemistry, Vol. 285, Issue 35, pp. 27130-43, (2010) (PubMed).

Target Details for GFP Tag

Target: GFP Tag

Target Type: Tag

Background: Green fluorescent protein (GFP), originally isolated from the jellyfish Aequorea victoria, is one of the best visual reporters for monitoring gene expression in vivo and in situ. GFP is a also convenient marker for use in flow cytometry because it eliminates the need to incubate with a secondary reagent (such as dyes or antibodies) for detection. However, anti-GFP antibody is also widely used for co-immunipreciapitation, co-localization or western blotting for the confirmation of specificity when a GFP fusion protein is expressed in cells. Abgent's anti-GFP monoclonal antibody provides a simple solution to detect the expression of a GFP-tagged protein in cells. Because of its ability to spontaneously generate its own fluorophore, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria is used extensively as a fluorescent marker in molecular and cell biology. The yellow fluorescent proteins (YFPs) have the longest wavelength emissions of all GFP variants examined to date. This shift in the spectrum is the result of a T203Y substitution (single-letter amino acid code), a mutation rationally designed on the basis of the X-ray structure of GFP S65T. Abgent's anti-GFP monoclonal antibody can detect both GFP and YFP but not BFP (Blue fluorescent protein) by western blotting.

Molecular Weight: 27 k

UniProt: C5MKY7