Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1) (C-Term), (AA 765-794) antibody

Details for Product No. ABIN390185
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Antigen
Epitope
C-Term, AA 765-794
(18), (5), (4), (2), (1), (1), (1), (1), (1)
Reactivity
Human
(59), (17), (16), (13), (12), (1), (1)
Host
Rabbit
(43), (16), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (IHC), Western Blotting (WB)
(42), (33), (21), (21), (12), (10), (10), (10), (9), (9), (1)
Pubmed 5 references available
Quantity 400 µL
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Catalog No. ABIN390185
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Immunogen This TAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 765-794 AA from the C-terminal region of human TAP1.
Clone RB1815-1816
Isotype Ig
Specificity This TAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773~802 amino acids from the C-terminal region of human TAP1.
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name TAP1
Background TAP is an integral transmembrane protein involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. It also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. TAP is inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. It is inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP and prevents the conformational rearrangement of TAP induced by peptide binding. TAP is also inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP is down-regulated by human Epstein-barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules. TAP1 and TAP2 form a heterodimer of TAP1 and TAP2, and the peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2. TAP, inducible by interferon gamma, belongs to the ABC transporter family, MDR subfamily.
Molecular Weight 80964 DA
Gene ID 6890
UniProt Q96CP4
Research Area Cell Structure
Application Notes IHC = 1:50-100, WB = 1:1000
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1) (C-Term), (AA 765-794) antibody Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
anti-Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1) (C-Term), (AA 765-794) antibody (2) TAP1 Antibody (I795) (ABIN390185) western blot analysis in Jurkat cell line lysates (35 µg/lane).This demonstrates the TAP1 antibody detected the TAP1 protein (arrow).
Background publications Bennett, Bennink, Yewdell et al.: "Cutting edge: adenovirus E19 has two mechanisms for affecting class I MHC expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 9, pp. 5049-52, 1999 (PubMed).

Hewitt, Gupta, Lehner: "The human cytomegalovirus gene product US6 inhibits ATP binding by TAP." in: The EMBO journal, Vol. 20, Issue 3, pp. 387-96, 2001 (PubMed).

Tang, Freedman, Allen et al.: "TAPI polymorphisms in several human ethnic groups: characteristics, evolution, and genotyping strategies." in: Human immunology, Vol. 62, Issue 3, pp. 256-68, 2001 (PubMed).

Gaudet, Wiley: "Structure of the ABC ATPase domain of human TAP1, the transporter associated with antigen processing." in: The EMBO journal, Vol. 20, Issue 17, pp. 4964-72, 2001 (PubMed).

Lajoie, Zijenah, Faucher et al.: "Novel TAP1 polymorphisms in indigenous Zimbabweans: their potential implications on TAP function and in human diseases." in: Human immunology, Vol. 64, Issue 8, pp. 823-9, 2003 (PubMed).

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