Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 305-335), (Center) antibody

Details for Product No. ABIN390189
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Antigen
Epitope
AA 305-335, Center
(7), (3), (1), (1)
Reactivity
Human
(27), (12), (12), (12)
Host
Rabbit
(26), (1)
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC)
(15), (12), (10), (9), (8), (1)
Pubmed 2 references available
Quantity 400 µL
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Catalog No. ABIN390189
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Immunogen This WISP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-335 AA from the Central region of human WISP3.
Clone RB2145-2146
Isotype Ig
Specificity This WISP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WISP3.
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name WISP3
Background WISP3 is a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. WISP3 is overexpressed in colon tumors. It may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. Mutations of the WISP3 gene are associated with progressive pseudorheumatoid dysplasia, an autosomal recessive skeletal disorder, indicating that the gene is essential for normal postnatal skeletal growth and cartilage homeostasis.
Synonyms: WNT1-inducible-signaling pathway protein 3, WISP-3, CCN6
Molecular Weight 39292 DA
Gene ID 8838
UniProt O95389
Research Area Cancer, Cell Cycle, Cell Structure
Application Notes WB = 1:1000, IHC = 1:50-100
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 305-335), (Center) antibody Western blot analysis of anti-WISP3 Antibody (Center) (ABIN390189) in HepG2 cell line lysates (35 µg/lane). WISP3 (arrow) was detected using the purified polyclonal antibody.
anti-Wnt1 Inducible Signaling Pathway Protein 3 (WISP3) (AA 305-335), (Center) antibody (2) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma
Background publications Pennica, Swanson, Welsh et al.: "WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and aberrantly expressed in human colon tumors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 25, pp. 14717-22, 1999 (PubMed).

Hurvitz, Suwairi, Van Hul et al.: "Mutations in the CCN gene family member WISP3 cause progressive pseudorheumatoid dysplasia." in: Nature genetics, Vol. 23, Issue 1, pp. 94-8, 1999 (PubMed).

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