EPH Receptor B2 (EPHB2) (N-Term), (AA 103-133) antibody

Details for Product No. ABIN391919
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Synonyms CAPB, DRT, EK5, EPHT3, ERK, Hek5, PCBC, Tyro5, Cek5, Drt, ETECK, Erk, Nuk, Prkm5, Qek5, Sek3, RGD1564232
N-Term, AA 103-133
(28), (18), (13), (9), (7), (7), (6), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Human, Mouse (Murine)
(129), (21), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
(72), (40), (21), (8)
Clonality (Clone)
Polyclonal ()
(10), (7), (7), (7), (7), (7), (2)
Immunohistochemistry (IHC)
(116), (88), (56), (30), (22), (21), (11), (9), (7), (5), (1), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 400 μL
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Catalog No. ABIN391919
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Immunogen This EphB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-133 AA from the N-terminal region of human EphB2.
Clone RB01650
Isotype Ig
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Alternative Name EphB2
Background Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.

Synonyms: Ephrin type-B receptor 2, Tyrosine-protein kinase receptor EPH-3, DRT, Receptor protein-tyrosine kinase HEK5, ERK
Molecular Weight 117506 Da
Gene ID 2048
UniProt P29323
Research Area Phospho-specific antibodies, Stem Cells, Cell Signaling, Protein Modifications, Cell Structure, Neurology
Application Notes Suggested dilutions:
IHC = 1:50-100
Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1.47 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots.
Expiry Date 6 months
Supplier Images
anti-EPH Receptor B2 (EPHB2) (N-Term), (AA 103-133) antibody Western blot analysis of hEPHB2-A118 (ABIN391919) in K562 cell line lysates (35 µg/lane). EPHB2 (arrow) was detected using the purified polyclonal antibody.
anti-EPH Receptor B2 (EPHB2) (N-Term), (AA 103-133) antibody (2) EphB2 Antibody (N-term) (ABIN391919)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Background publications Thanos, Goodwill, Bowie: "Oligomeric structure of the human EphB2 receptor SAM domain." in: Science (New York, N.Y.), Vol. 283, Issue 5403, pp. 833-6, 1999 (PubMed).

Ikegaki, Tang, Liu et al.: "Molecular characterization and chromosomal localization of DRT (EPHT3): a developmentally regulated human protein-tyrosine kinase gene of the EPH family." in: Human molecular genetics, Vol. 4, Issue 11, pp. 2033-45, 1996 (PubMed).

Iwase, Tanaka, Suzuki et al.: "Identification of protein-tyrosine kinase genes preferentially expressed in embryo stomach and gastric cancer." in: Biochemical and biophysical research communications, Vol. 194, Issue 2, pp. 698-705, 1993 (PubMed).

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