Tiparp antibody (N-Term)

Catalog No. ABIN404897

The Rabbit Polyclonal anti-Tiparp antibody has been validated for WB. It is suitable to detect Tiparp in samples from Human, Mouse, Rat, Cow, Dog, Horse, Pig and Rabbit. There are 2+ publications available.

Quick Overview for Tiparp antibody (N-Term) (ABIN404897)

Target: Tiparp (TCDD-Inducible Poly(ADP-Ribose) Polymerase (Tiparp))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tiparp antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Tiparp Antibody

Binding Specificity: N-Term

Sequence: LKTCFKKKDQ KRLGTGTLRS LRPILNTLLE SGSLDGVFRS RNQSTDENSL

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 83%, Pig: 100%, Rabbit: 92%, Rat: 83%

Characteristics: This is a rabbit polyclonal antibody against TIPARP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TIPARP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 657 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Tiparp antibody (ABIN404897)

MacPherson, Tamblyn, Rajendra, Bralha, McPherson, Matthews: "2,3,7,8-Tetrachlorodibenzo-p-dioxin poly(ADP-ribose) polymerase (TiPARP, ARTD14) is a mono-ADP-ribosyltransferase and repressor of aryl hydrocarbon receptor transactivation." in: Nucleic acids research, Vol. 41, Issue 3, pp. 1604-21, (2013) (PubMed).

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for Tiparp

Target: Tiparp (TCDD-Inducible Poly(ADP-Ribose) Polymerase (Tiparp))

Alternative Name: TIPARP

Background: TIPARP is a poly [ADP-ribose] polymerase using NAD+ as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor, repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. TIPARP may play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
Alias Symbols: DDF1, DKFZP434J214, DKFZp686N0351, DKFZp686P1838, FLJ40466, PARP-1, PARP-7, PARP7, pART14
Protein Interaction Partner: UBC, XRN2, ATM,
Protein Size: 657

Molecular Weight: 76 kDa

Gene ID: 25976

NCBI Accession: NM_015508, NP_056323

UniProt: Q7Z3E1

Pathways: Platelet-derived growth Factor Receptor Signaling