MARCH4 antibody (N-Term)

Catalog No. ABIN405545

The Rabbit Polyclonal anti-MARCH4 antibody has been validated for WB. It is suitable to detect MARCH4 in samples from Human, Mouse, Rat, Dog, Horse, Guinea Pig and Rabbit.

Quick Overview for MARCH4 antibody (N-Term) (ABIN405545)

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Reactivity: Human, Mouse, Rat, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCH4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCH4 Antibody

Binding Specificity: N-Term

Sequence: GLLKCRCRML FNDLKVFLLR RPPQAPLPMH GDPQPPGLAA NNTLPALGAG

Predicted Reactivity: Dog: 92%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against MARCH4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MARCH4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 410 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MARCH4

Target: MARCH4 (Membrane-Associated Ring Finger (C3HC4) 4, E3 Ubiquitin Protein Ligase (MARCH4))

Alternative Name: MARCH4

Background: MARCH4 is an E3 ubiquitin-protein ligase that may mediate ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates.
Alias Symbols: MARCH-IV, MGC104908, RNF174
Protein Interaction Partner: UBE2H, UBE2D1, ULBP1, CD4,
Protein Size: 410

Molecular Weight: 45 kDa

Gene ID: 57574

NCBI Accession: NM_020814, NP_065865

UniProt: Q9P2E8