ACTA2 / Aortic Smooth Muscle Actin (N-Term) antibody

Details for Product No. ABIN452371
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Antigen
Epitope
N-Term
(17), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Chicken, Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(27), (16), (16), (13), (13), (13), (5), (5), (1)
Host
Mouse
(18), (12), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(27), (17), (9), (9), (6), (2), (1)
Pubmed 4 references available
Quantity 5 mL
Shipping to United States (Change)
Availability Will be delivered in 6 to 8 Business Days
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Catalog No. ABIN452371
308.00 $
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Immunogen Synthetic N- terminus decapeptide of alpha-smooth-muscleisoform of Actin.
Clone ASM-1
Isotype IgG2a
Purification Affinity Chromatography on Protein A
Background The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actinand myosin are constituents of many cell types and are involved in a myriad of cellularprocesses including locomotion, secretion, cytoplasmic streaming, phagocytosis andcytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresisand amino acid sequence analysis. Four of them represent the differentiation markers ofmuscle tissues and two are found in practically all cells.
Gene ID 58
UniProt P68133
Application Notes Immunofluorescence MicroscopyImmunoblotting (Western). Cytological Material. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pretreatment is required prior toantibody application. Working Dilution: Ready-to-use for Immunohistochemical application. Incubation Time: 1 h at RT, extended with paraffin. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4 0.09% Sodium Azide 0.5% BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluited at 2-8°CShelf life: One year from despatch.
Expiry Date 12 months
Background publications Mason: "A new look at lymphoma immunohistology." in: The American journal of pathology, Vol. 128, Issue 1, pp. 1-4, 1987 (PubMed).

Skalli, Ropraz, Trzeciak et al.: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Ehler, Fowler, Perriard: "Myofibrillogenesis in the developing chicken heart: role of actin isoforms and of the pointed end actin capping protein tropomodulin during thin filament assembly." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 229, Issue 4, pp. 745-55, 2004 (PubMed).

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