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ACTA2 / Aortic Smooth Muscle Actin (N-Term) antibody

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(11), (1), (1)
Chicken, Cow (Bovine), Horse (Equine), Human, Mouse (Murine), Rat (Rattus)
(18), (11), (11), (8), (8), (8), (5), (1), (1)
(9), (7), (2)
Clonality (Clone)
Monoclonal ()
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(16), (9), (6), (6), (2), (1), (1)
Pubmed 4 references available
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Quantity 5 mL
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic N- terminus decapeptide of alpha-smooth-muscleisoform of Actin.
Clone ASM-1
Isotype IgG2a
Specificity ASM-1 represents an excellent marker for myogenic soft tissue tumors and smooth muscle differentiation. Polypeptide reacting: specific for alpha-smooth-muscle isoform of actin (Mr 43,000). Tumors Specificallly Detected: leiomyosarcoma, leiomyoma, certain stromal cells surrounding infiltrating ductal carcinoma of breast. Tested Reactivities on Cultured Cell lines: Stress fibers of smooth muscle-derived cells and some smooth muscle subtype fibroblasts.
Cross-Reactivity (Details) Species reactivity (tested):Human, Bovine, Equine, Rat, Mouse and Chicken.
Purification Affinity Chromatography on Protein A
Background The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis and cytokinesis. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as at least six isoforms characterized by electrophoresis and amino acid sequence analysis. Four of them represent the differentiation markers of muscle tissues and two are found in practically all cells.Synonyms: ACTSA, ACTVS, Alpha-actin-2, Cell growth-inhibiting gene 46 protein
Gene ID 58
UniProt P68133
Application Notes Immunofluorescence MicroscopyImmunoblotting (Western). Cytological Material. Immunohistochemistry on Frozen and Paraffin-Embedded Tissue. Pretreatment: With paraffin embedded sections, protease pretreatment is required prior toantibody application. Working Dilution: Ready-to-use for Immunohistochemical application. Incubation Time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS, pH 7.4, 0.09 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluited at 2-8 °CShelf life: One year from despatch.
Expiry Date 12 months
Background publications Ehler, Fowler, Perriard: "Myofibrillogenesis in the developing chicken heart: role of actin isoforms and of the pointed end actin capping protein tropomodulin during thin filament assembly." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 229, Issue 4, pp. 745-55, 2004 (PubMed).

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996 (PubMed).

Skalli, Ropraz, Trzeciak et al.: "A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation." in: The Journal of cell biology, Vol. 103, Issue 6 Pt 2, pp. 2787-96, 1987 (PubMed).

Mason: "A new look at lymphoma immunohistology." in: The American journal of pathology, Vol. 128, Issue 1, pp. 1-4, 1987 (PubMed).

Catalog No. ABIN452371
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