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Details for Product No. ABIN487054

Breast Cancer 1 (BRCA1) (Middle Region) antibody

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Antigen
Synonyms BRCA1
Epitope
»Alternatives Middle Region
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN487054
Quantity 50 µg
Price
289.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Immunogen The immunogen for anti-BRCA1 antibody: synthetic peptide directed towards the middle region of human BRCA1
Sequence DDLLDDGEIKEDTSFAENDIKESSAVFSKSVQKGELSRSP SPFTHTHLAQ
Predicted Reactivity Human : 100%
Characteristics This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot using a cell lysate as a positive control. We strive to provide antibodies covering each member of a whole protein family of your interest.
Purification Affinity Purified
Alternative Name BRCA1
Background The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
Molecular Weight 208kDa
Gene ID 672
NCBI Accession NP_009225, NM_007294
UniProt P38398
Research Area Cancer, Transcription Factors, Proteolysis / Ubiquitin, DNA/RNA
Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water
Concentration 1mg/mL
Buffer Final anti-BRCA1 antibody concentration is 1 mg/mL in PBS buffer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Product cited in: Evans, Shenton, Woodward et al.: "Penetrance estimates for BRCA1 and BRCA2 based on genetic testing in a Clinical Cancer Genetics service setting: risks of breast/ovarian cancer quoted should reflect the cancer burden in the family." in: BMC cancer, Vol. 8, pp. 155, 2008 (PubMed).

Alternatives for antigen "Breast Cancer 1 (BRCA1)", type "Antibodies"
Hosts (294), (81), (4), (1)
Reactivities (357), (35), (24), (13), (2), (2), (1), (1), (1), (1)
Applications (217), (96), (90), (84), (73), (69), (63), (4), (2), (2), (1), (1), (1)
Conjugates (16), (16), (10), (9), (9), (9), (9), (9), (9), (9), (9), (6), (3), (3), (1)
Epitopes (37), (33), (24), (17), (16), (15), (15), (14), (13), (12), (12), (7), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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