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BRCA1 antibody (Middle Region)

BRCA1 Reactivity: Human, Rat, Mouse, Cow WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN487054
  • Target See all BRCA1 Antibodies
    BRCA1 (Breast Cancer 1 (BRCA1))
    Binding Specificity
    • 22
    • 18
    • 15
    • 15
    • 14
    • 11
    • 10
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 193
    • 36
    • 25
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Cow
    Host
    • 162
    • 37
    • 2
    Rabbit
    Clonality
    • 166
    • 35
    Polyclonal
    Conjugate
    • 94
    • 18
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This BRCA1 antibody is un-conjugated
    Application
    • 127
    • 67
    • 42
    • 42
    • 41
    • 37
    • 29
    • 24
    • 22
    • 12
    • 12
    • 10
    • 9
    • 9
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Sequence
    DDLLDDGEIK EDTSFAENDI KESSAVFSKS VQKGELSRSP SPFTHTHLAQ
    Predicted Reactivity
    Cow: 75%, Human: 100%, Mouse: 79%, Rat: 86%
    Characteristics
    This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human BRCA1
    Top Product
    Discover our top product BRCA1 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 1863 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Evans, Shenton, Woodward, Lalloo, Howell, Maher: "Penetrance estimates for BRCA1 and BRCA2 based on genetic testing in a Clinical Cancer Genetics service setting: risks of breast/ovarian cancer quoted should reflect the cancer burden in the family." in: BMC cancer, Vol. 8, pp. 155, (2008) (PubMed).

  • Target
    BRCA1 (Breast Cancer 1 (BRCA1))
    Alternative Name
    BRCA1 (BRCA1 Products)
    Synonyms
    BRCAI antibody, BRCC1 antibody, BROVCA1 antibody, IRIS antibody, PNCA4 antibody, PPP1R53 antibody, PSCP antibody, RNF53 antibody, iris antibody, pscp antibody, brcai antibody, brcc1 antibody, rnf53 antibody, brca1 antibody, BRCA1 antibody, LOC100224649 antibody, BRCA1, DNA repair associated antibody, breast cancer 1 antibody, breast cancer 1, early onset antibody, breast cancer 1 L homeolog antibody, BRCA1 antibody, brca1 antibody, LOC755086 antibody, Brca1 antibody, brca1.L antibody
    Background
    The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability and acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as BASC for BRCA1-associated genome surveillance complex. This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complex. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40 % of inherited breast cancers and more than 80 % of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants have been described for this gene but only some have had their full-length natures identified.
    Alias Symbols: BRCAI, BRCC1, IRIS, PSCP, RNF53, PNCA4, BROVCA1, PPP1R53
    Protein Interaction Partner: RAD50, MED17, RAD51, YAP3, HDA3, SSN3, BEM4, GYP5, YAF9, HDAC2, MSH6, BAP1, SUPT5H, POLR2A, NPR1, NUP53, MET18, CDC21, MLP1, CTK1, MSH2, BACH1, DAN1, TMA22, BBC1, RPB4, SPT4, YGR053C, ATE1, YBP2, SLI15, RAD16, XRS2, RAD55, BUR2, BRIP1, ESR1, BECN1, HIST1H
    Protein Size: 1863
    Molecular Weight
    208 kDa
    Gene ID
    672
    NCBI Accession
    NM_007294, NP_009225
    UniProt
    P38398
    Pathways
    Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus
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