F4/80 antibody

Catalog No. ABIN488117

The Rat Monoclonal anti-F4/80 antibody has been validated for FACS, WB, IHC (p), IP, IHC (fro), RIA and IEM. It is suitable to detect F4/80 in samples from Mouse. There are 9+ publications available.

Quick Overview for F4/80 antibody (ABIN488117)

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This F4/80 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA), Immunoelectron Microscopy (IEM)

Clone: Cl:A3-1

Product Details anti-F4/80 Antibody

Specificity: This antibody recognises the F4/80 antigen, a member of the EGF-TM7 family of proteins which shares 68 % overall amino acid identity with Human EMR1. Clone CI:A31 has been reported to modulate cytokine levels released in response to Listeria monocytogenes (Ref.5). We recommend the use of BM4008LE for this purpose.

Cross-Reactivity (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography on Protein G

Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice. Spleen cells from immunised HOB2 rats were fused with cells of the mouse NS1 myeloma cell line.

Isotype: IgG2b

Application Details

Application Notes: RIA. Western Blot. Immunoprecipitation. Flow Cytometry: Use 10 μL of 1/50-1/100 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen and Paraffin Embedded and Resin Sections. Thisproduct requires pre-treatment of paraffin sections (Proteinase K is recommended fortissues fixed for less than 24 hours. Citrate buffer pH 6.0 is recommended for tissues fixedfor more than 24 hours).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-F4/80 antibody (ABIN488117)

Schindewolffs, Breves, Buettner, Hadamitzky, Pabst: "VEGF-C improves regeneration and lymphatic reconnection of transplanted autologous lymph node fragments: An animal model for secondary lymphedema treatment." in: Immunity, inflammation and disease, Vol. 2, Issue 3, pp. 152-61, (2014) (PubMed).

Cuzi?, Bosnar, Kramari?, Ferenci?, Markovi?, Glojnari?, Erakovi? Haber: "Claudin-3 and Clara cell 10 kDa protein as early signals of cigarette smoke-induced epithelial injury along alveolar ducts." in: Toxicologic pathology, Vol. 40, Issue 8, pp. 1169-87, (2012) (PubMed).

Nakao, Zandi, Faez, Kohno, Hafezi-Moghadam: "Discontinuous LYVE-1 expression in corneal limbal lymphatics: dual function as microvalves and immunological hot spots." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 26, Issue 2, pp. 808-17, (2012) (PubMed).

Weisel, Appelt, Schneider, Horlitz, van Rooijen, Korner, Mach, Winkler: "Unique requirements for reactivation of virus-specific memory B lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4011-21, (2010) (PubMed).

Hakroush, Moeller, Theilig, Kaissling, Sijmonsma, Jugold, Akeson, Traykova-Brauch, Hosser, Hähnel, Gröne, Koesters, Kriz: "Effects of increased renal tubular vascular endothelial growth factor (VEGF) on fibrosis, cyst formation, and glomerular disease." in: The American journal of pathology, Vol. 175, Issue 5, pp. 1883-95, (2010) (PubMed).

Spyridonidou, Fousteris, Antonia, Chatzianastasiou, Papapetropoulos, Nikolaropoulos: "Tricyclic indole and dihydroindole derivatives as new inhibitors of soluble guanylate cyclase." in: Bioorganic & medicinal chemistry letters, Vol. 19, Issue 16, pp. 4810-3, (2009) (PubMed).

Kimura, Miyashita, Suzuki, Kobayashi, Watanabe, Sonoda, Ohta, Fujiwara, Shimosegawa, Sato: "Distinctive localization and opposed roles of vasohibin-1 and vasohibin-2 in the regulation of angiogenesis." in: Blood, Vol. 113, Issue 19, pp. 4810-8, (2009) (PubMed).

Kakehashi, Nishioku, Tsukuba, Kadowaki, Nakamura, Yamamoto: "Differential regulation of the nature and functions of dendritic cells and macrophages by cathepsin E." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 9, pp. 5728-37, (2007) (PubMed).

Anghelina, Krishnan, Moldovan, Moldovan: "Monocytes/macrophages cooperate with progenitor cells during neovascularization and tissue repair: conversion of cell columns into fibrovascular bundles." in: The American journal of pathology, Vol. 168, Issue 2, pp. 529-41, (2006) (PubMed).

Target Details for F4/80

Target: F4/80 (EMR1) (Egf-Like Module Containing, Mucin-Like, Hormone Receptor-Like 1 (EMR1))

Alternative Name: Macrophage F4/80 Antigen

Background: F4/80 antigen is a 160 kD glycoprotein expressed by most murine macrophages. Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymphnode. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.Synonyms: Cell surface glycoprotein EMR1, EMR1 hormone receptor, Emr1, Gpf480

Gene ID: 13733

NCBI Accession: NP_034260

UniProt: Q61549