TNFRSF8 antibody (FITC)

Catalog No. ABIN492586

Product Details anti-TNFRSF8 Antibody (FITC)

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNFRSF8 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Specificity: This antibody reacts with CD30 on Flow Cytometry.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: TNFRSF8, D1S166E, CD30L receptor, KI-1 antigen, Tumor necrosis factor receptorsuperfamily member 8, Lymphocyte activation antigen CD30

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Co cell line cellsRemarks: Hybridoma was established by fusion of mouse myeloma cell with Balb/c mousesplenocyte

Clone: Ber-H2

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 5-10 μg/mL (final concentration). Detailed procedure is provided in Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow cytometric analysis for floating cellsWe usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% NaN3]. 2) Resuspend the cells with washing buffer (5x10e6 cells/mL). 3) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atRT (20~25°C). Remove supernatant by careful aspiration. 4) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3or 20 µL of Clear Back (human Fc receptor blocking reagent) to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 20 µL of the FITC labeled CD30 monoclonal antibody (Ber-H2) (5-10 µg/mL) dilutedwith the washing buffer. Mix well and incubate for 30 minutes at RT (20~25°C). 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at RT. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control: CCRF-CEMFlow cytometric analysis for whole blood cellsWe usually use Falcon tubes or equivalents as reaction tubes for all steps described below. 1) Add 20 µL of the FITC labeled CD163 monoclonal antibody (Ber-Mac3) (5-10 µg/mL)diluted with the washing buffer [PBS containing 2% fetal calf serum(FCS) and 0. 1% NaN3] into each tube. 2) Add 50 µL of whole blood into each tube. Mix well, and incubate for 30 minutes at RT(20~25°C). 3) Lyse with OptiLyse C (for analysis on Beckman Coulter instruments) or OptiLyse B (foranalysis on BD instruments), using the procedure recommended in the respective packageinserts. 4) Add 1mL of H2O to each tube and incubate for 10 minutes at RT (20~25°). 5) Centrifuge at 500 x g for 1 minute at RT (20~25°C). Remove supernatant by carefulaspiration. 6) Add 1 mL of washing buffer followed by centrifugation at 500 x g for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer.

Restrictions: For Research Use only

Handling

Concentration: 50 µg/mL

Buffer: PBS containing 5 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TNFRSF8

Target: TNFRSF8 (Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8))

Alternative Name: CD30 (TNFRSF8 Products)

Background: CD30, also known as Ki-1, TNFRSF8, or Be-H2, is a 120 kDa glycoprotein expressed on the surface of mitogen-activated B-cells and T-cells but not on resting lymphocytes or monocytes. CD30 is also a marker for Hodgkin and Sternberg-Reed cells of Hodgkin's lymphomas and related hematologic malignancies. Soluble forms of CD30 have been found in the serum of patients with adult T-cell leukemia or other CD30+ lymphomas. The CD30 ligand, CD153, is a type II transmembrane glycoprotein that enhances proliferation of activated T-cells and induces apoptosis in CD30+ lymphoma-derived cell lines.Synonyms: CD30L receptor, D1S166E, KI-1 antigen, Lymphocyte activation antigen CD30, TNFRSF8, Tumor necrosis factor receptor superfamily member 8

Gene ID: 943

UniProt: P28908