TNFRSF8 antibody (Tumor Necrosis Factor Receptor Superfamily, Member 8)

Details for Product anti-TNFRSF8 Antibody No. ABIN94067
Antigen
Reactivity
Human
406
80
19
Host
Mouse
260
161
41
5
3
1
Clonality (Clone)
Monoclonal ()
Conjugate
This TNFRSF8 antibody is un-conjugated
33
27
23
13
6
6
6
6
6
6
6
6
6
6
6
6
6
5
3
3
3
3
3
3
3
2
2
2
2
2
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
304
172
150
101
84
54
37
26
19
10
9
5
2
1
1
1
1
Options
Immunogen Expression vector containing CD30 cDNA (booster suspension of THP-1 cell line)
Clone MEM-268
Isotype IgG
Specificity The antibody MEM-268 recognizes extracellular part of CD30 (Ki-1 antigen), a 105 kDa single chain glycoprotein expressed on Hodgkin's and Reed-Sternberg cells, it is also found in Burkitt's lymphomas, virus-infected T and B lymphocytes, and on normal B and T lymphocytes after activation (T lymphocytes that produce Th2-type cytokines and on CD4+/CD8+ T lymphocytes that co-express CD45RO and the IL4 receptor).
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD30 (TNFRSF8 Antibody Abstract)
Background CD30 is a type I transmembrane glycoprotein of the TNF receptor superfamily. CD30 was originally identified as a cell surface antigen of Hodgkins and Reed-Sternberg cells using monoclonal antibody Ki-1. The ligand for CD30 is CD30L (CD153). The binding of CD30 to CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation, and apoptotic cell death. CD30 has a critical role in the pathophysiology of Hodgkin's disease and other CD30+ lymphomas. CD30 acts as a costimulatory molecule in thymic negative selection. In addition to its expression on Hodgkin's and Reed-Sternberg cells, CD30 is also found in some non-Hodgkin's lymphomas (including Burkitt's lymphomas), virus-infected T and B cells, and on normal T and B cells after activation. In T cells, CD30 expression is present on a subset of T cells that produce Th2-type cytokines and on CD4+/CD8+ thymocytes that co-express CD45RO and the IL4 receptor. Soluble form of CD30 (sCD30) serves as a marker reflecting Th2 immune response.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8) antibody (ABIN94067) Human Thymus (formalin-fixed, paraffin-embedded) stained with TNFRSF8 antibody LS-B18...
Product cited in: Pavlov, Martins, Delgado: "Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing." in: Clinical and vaccine immunology : CVI, Vol. 16, Issue 9, pp. 1327-31, 2009 (PubMed).