NDUFS8 antibody (Internal Region)

Catalog No. ABIN499135

This Goat Polyclonal antibody specifically detects NDUFS8 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for NDUFS8 antibody (Internal Region) (ABIN499135)

Target: NDUFS8 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 8, 23kDa (NADH-Coenzyme Q Reductase) (NDUFS8))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NDUFS8 Antibody

Binding Specificity: Internal Region

Sequence: C-EAEIAANIQA D

Specificity: Recognizes NDUFS8.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002487.1. Genename: NDUFS8

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/4000. Western blot: 0.1-0.3 μg/mL. This antibody detects a band of approx. 25 kDa in Mouse and RatHeart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDUFS8

Target: NDUFS8 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 8, 23kDa (NADH-Coenzyme Q Reductase) (NDUFS8))

Alternative Name: NDUFS8

Background: NDUFS8 is the core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (may donate electrons to ubiquinone). Defects in NDUFS8 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions.Synonyms: CI-23kD, Complex I-23kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY subunit, mitochondrial

Molecular Weight: 24.0kDa (Mouse NP_659119.2 and Rat NP_001099792.1).

Gene ID: 4728

NCBI Accession: NP_002487

UniProt: O00217