AICDA antibody (C-Term)

Catalog No. ABIN499233

Cited in 1 publication. The Rabbit Polyclonal anti-AICDA antibody (ABIN499233) specifically detects AICDA in WB, IF and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for AICDA antibody (C-Term) (ABIN499233)

Target: AICDA (Activation-Induced Cytidine Deaminase (AICDA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AICDA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-AICDA Antibody

Binding Specificity: C-Term

Specificity: This antibody recognizes AID (C-term).

Purification: Affinity chromatography purified via peptide column

Immunogen: A Peptide corresponding to 14 amino acids near the carboxy-terminus of Human AID

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: AID antibody can be used for detection of AID at 1 - 2 μg/mL. Whole cell lysatefrom Ramos cells can be used as positive control. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-AICDA antibody (ABIN499233)

Milillo, Molinario, Costanzi, Vischini, La Carpia, La Greca, Rigante, Gambaro, Gurrieri, Sangiorgi: "Defective activation of the MAPK/ERK pathway, leading to PARP1 and DNMT1 dysregulation, is a common defect in IgA nephropathy and Henoch-Schönlein purpura." in: Journal of nephrology, Vol. 31, Issue 5, pp. 731-741, (2018) (PubMed).

Target Details for AICDA

Target: AICDA (Activation-Induced Cytidine Deaminase (AICDA))

Alternative Name: AICDA / AID

Background: Activation-induced cytidine deaminase (AID) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines (1). It is necessary for somatic hypermutation and class switch recombination in B cells (2), but inappropriate or dysregulated expression AID is often found in tumors and B cell neoplasms (2,3). Although it is structurally and functionally similar to the APOBEC proteins (reviewed in 4), it appears unlikely that AID deaminates dC to dU residues in HIV cDNA as does APOBEC3G (5).Synonyms: Activation-induced cytidine deaminase, Cytidine aminohydrolase

Gene ID: 57379

NCBI Accession: NP_065712

UniProt: Q9GZX7

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response