AICDA antibody (AA 178-198)

Catalog No. ABIN2668532

Cited in 1 publication. The Rat Monoclonal anti-AICDA antibody (Clone 328-8b) (ABIN2668532) specifically detects AICDA in WB and ChIP. The antibody is reactive with Human and Mouse samples.

Quick Overview for AICDA antibody (AA 178-198) (ABIN2668532)

Target: AICDA (Activation-Induced Cytidine Deaminase (AICDA))

Reactivity: Human, Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This AICDA antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP)

Clone: 328-8b

Product Details anti-AICDA Antibody

Binding Specificity: AA 178-198

Purpose: AID antibody (mAb) (Clone 328.8b)

Purification: Protein G Chromatography

Immunogen: This AID antbody was raised against a peptide corresponding to amino acids 178-198 of human AID.

Isotype: IgG2a

Application Details

Application Notes: WB: 1 - 3 μg/mL dilution

Restrictions: For Research Use only

Handling

Concentration: 1 μg/μL

Buffer: Purified IgG in 50 mM Tris-HCl ( pH 7.2), 30 % glycerol, and 0.035 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Storage: -20 °C

Storage Comment: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Expiry Date: 24 months

References for anti-AICDA antibody (ABIN2668532)

Cortizas, Zahn, Hajjar, Patenaude, Di Noia, Verdun: "Alternative end-joining and classical nonhomologous end-joining pathways repair different types of double-strand breaks during class-switch recombination." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 191, Issue 11, pp. 5751-63, (2013) (PubMed).

Target Details for AICDA

Target: AICDA (Activation-Induced Cytidine Deaminase (AICDA))

Alternative Name: AID

Background: AID (Activation-induced cytidine deaminase, AICDA) is a member of the cytidine and deoxycytidylate deaminase family. It acts act as a single-strand DNA deaminase involved in somatic hypermutation, gene conversion and class-switch recombination in developing B-cells and is necessary for efficient antibody responses. It has also been implicated in active DNA demethylation, as deamination of methylcytosine yields thymine, which is read as a mismatch and is replaced by DNA damage repair enzymes.

Molecular Weight: 24 kDa

Gene ID: 57379

NCBI Accession: NP_065712

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Production of Molecular Mediator of Immune Response