MADD antibody (C-Term)

Catalog No. ABIN500216

The Rabbit Polyclonal anti-MADD antibody has been validated for WB, IF and EIA. It is suitable to detect MADD in samples from Human, Mouse and Rat.

Quick Overview for MADD antibody (C-Term) (ABIN500216)

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MADD antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-MADD Antibody

Binding Specificity: C-Term

Specificity: This antibody detects MADD at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, mouse, rat

Purification: DEAE purified

Immunogen: MADD antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human MADD.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 1: 250 - 1: 500 dilution, 200 to 220 kDa bands should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for MADD

Target: MADD (MAP-Kinase Activating Death Domain (MADD))

Alternative Name: MADD

Background: MAP kinase-activating death domain protein (MADD) was initially identified as the type 1 tumor necrosis factor receptor (TNFR1) associated protein though their death domains. Overexpression of MADD activates MAP kinases ERK and JNK and induces the phosphorylation of cytosolic phospholipase A2. MADD shares 98 % identity with DENN (for differentially expressed in neoplastic vs. normal cells), which was recently identified as a substrate for c-jun N-terminal kinase 3 (JNK3). MADD has greater than 94 % overall identity to a GDP/GTP exchange protein Rab3-GEP. MADD is 87 % identical to KIAA0358, a brain protein of unknown function. Identification of MADD as a component of the TNFR1 signaling complex and the similarity between MADD and Rab3-GEP provides a connection between TNFR1 activation and downstream MAP kinase activity through a guanine-nucleotide exchange protein.Synonyms: DENN, IG20, MAP kinase-activating death domain protein, RAB3GEP, Rab3 GDP/GTP exchange factor

Gene ID: 8567

UniProt: Q8WXG6

Pathways: Caspase Cascade in Apoptosis