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TAR DNA Binding Protein (TARDBP) (Center) antibody

Details for Product No. ABIN500883, Supplier: Login to see
Antigen
  • TARDBP
  • TDP43
  • DKFZp459I2127
  • tardbp
  • ALS10
  • TDP-43
  • 1190002A23Rik
  • C85084
  • Tdp43
  • wu:fb77f02
  • wu:fc52g10
Epitope
Center
28
22
16
14
13
12
12
8
7
4
3
3
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
163
67
53
10
8
8
7
6
5
4
3
2
1
1
Host
Rabbit
120
34
15
1
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
5
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
149
73
54
40
34
20
12
11
5
4
3
3
2
1
Supplier
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Immunogen TDP43 antibody was raised against a 18 amino acid peptide from near the center of human TDP43.
Isotype IgG
Specificity At least two isoforms are known to exist for this protein, this TDP43 antibody only recongizes the longer of the two isoforms.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse, rat
Purification Peptide affinity chromatography
Alternative Name TARDBP (TARDBP Antibody Abstract)
Background TDP43 was initially identified as a novel cellular protein that bound to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. Later experiments revealed that TDP43 also regulates the splicing of exon 9 of the cystic fibrosis transmembrane conductance regular (CFTR), most likely through the association with the UG repeats at the 3' splice site. A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences. At least two isoforms are known to exist for this protein, this TDP43 antibody only recongizes the longer of the two isoforms.Synonyms: TAR DNA-binding protein 43, TDP-43, TDP43
Gene ID 23435
NCBI Accession NP_031401
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Pathways
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL. Immunoflourescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Immunofluorescence (IF) image for anti-TAR DNA Binding Protein (TARDBP) (Center) antibody (ABIN500883) Immunocytochemistry of TDP43 in HeLa cells with this product at 5 μg/ml.
Western Blotting (WB) image for anti-TAR DNA Binding Protein (TARDBP) (Center) antibody (ABIN500883) Western blot analysis of TDP43 in L1210 cell lysate with this product at (A) 0.5, (B)...
Background publications Neumann, Sampathu, Kwong et al.: "Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis." in: Science (New York, N.Y.), Vol. 314, Issue 5796, pp. 130-3, 2006 (PubMed).

Buratti, Dörk, Zuccato et al.: "Nuclear factor TDP-43 and SR proteins promote in vitro and in vivo CFTR exon 9 skipping." in: The EMBO journal, Vol. 20, Issue 7, pp. 1774-84, 2001 (PubMed).

Ou, Wu, Harrich et al.: "Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs." in: Journal of virology, Vol. 69, Issue 6, pp. 3584-96, 1995 (PubMed).