TARDBP antibody (N-Term)

Catalog No. ABIN2780848

There is 1 publication for this product available. The Rabbit Polyclonal anti-TARDBP antibody is suitable to detect TARDBP in samples from Human, Mouse, Rat, Cow, Dog, Horse and Guinea Pig. It has been validated for WB and IHC.

Quick Overview for TARDBP antibody (N-Term) (ABIN2780848)

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TARDBP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TARDBP Antibody

Binding Specificity: N-Term

Purpose: TARDBP Antibody - N-terminal region

Sequence: MSEYIRVTED ENDEPIEIPS EDDGTVLLST VTAQFPGACG LRYRNPVSQC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TARDBP. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TARDBP

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8113485

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-TARDBP antibody (ABIN2780848)

Estes, Boehringer, Zwick, Tang, Grigsby, Zarnescu: "Wild-type and A315T mutant TDP-43 exert differential neurotoxicity in a Drosophila model of ALS." in: Human molecular genetics, Vol. 20, Issue 12, pp. 2308-21, (2011) (PubMed).

Target Details for TARDBP

Target: TARDBP (TAR DNA Binding Protein (TARDBP))

Alternative Name: TARDBP

Background:

Background Information: HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. TARDBP is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription.HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20.

Gene Name: TAR DNA binding protein

Alternative Symbols: ALS10, TDP-43

Protein Name: TAR DNA-binding protein 43

Molecular Weight: 45kDa

Gene ID: 23435

NCBI Accession: NP_031401

UniProt: Q13148

Pathways: Positive Regulation of Peptide Hormone Secretion