TRPV4 antibody (Middle Region)

Catalog No. ABIN501411

This anti-TRPV4 antibody is a Rabbit Polyclonal antibody detecting TRPV4 in WB and IHC. Suitable for Human, Rat, Mouse, Cow, Pig, Dog, Rabbit and Guinea Pig. This Primary Antibody has been cited in 1 publication.

Quick Overview for TRPV4 antibody (Middle Region) (ABIN501411)

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Reactivity: Human, Rat, Mouse, Cow, Pig, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRPV4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-TRPV4 Antibody

Binding Specificity: Middle Region

Sequence: RVDEVNWSHW NQNLGIINED PGKNETYQYY GFSHTVGRLR RDRWSSVVPR

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against TRPV4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TRPV4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 811 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TRPV4 antibody (ABIN501411)

Sampat, Dermksian, Oungoulian, Winchester, Bulinski, Ateshian, Hung: "Applied osmotic loading for promoting development of engineered cartilage." in: Journal of biomechanics, Vol. 46, Issue 15, pp. 2674-81, (2013) (PubMed).

Target Details for TRPV4

Target: TRPV4 (Transient Receptor Potential Cation Channel, Subfamily V, Member 4 (TRPV4))

Alternative Name: TRPV4

Background: TRPV4 is a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. TRPV4 is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure.This gene encodes a member of the OSM9-like transient receptor potential channel (OTRPC) subfamily in the transient receptor potential (TRP) superfamily of ion channels. The encoded protein is a Ca2+-permeable, nonselective cation channel that is thought to be involved in the regulation of systemic osmotic pressure. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: OTRPC4, TRP12, VR-OAC, VRL-2, VRL2, VROAC, CMT2C, SPSMA, HMSN2C, SSQTL1
Protein Interaction Partner: UBC, ARRB2, KRIT1, ITCH, PACSIN3, PACSIN1, TRPV4, PACSIN2, MAP7, SRC, LYN, YES1, LCK, HCK, FYN, CALM1, OS9, PACS1,
Protein Size: 811

Molecular Weight: 91 kDa

Gene ID: 59341

NCBI Accession: NM_147204, NP_671737

Pathways: Hormone Transport, Cell-Cell Junction Organization