UBA1 antibody (N-Term)

Catalog No. ABIN504512

The Rabbit Polyclonal anti-UBA1 antibody has been validated for WB. It is suitable to detect UBA1 in samples from Human, Rat, Mouse, Dog, Cow, Horse, Rabbit and Guinea Pig. There is 1 publication available.

Quick Overview for UBA1 antibody (N-Term) (ABIN504512)

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Reactivity: Human, Rat, Mouse, Dog, Cow, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-UBA1 Antibody

Binding Specificity: N-Term

Sequence: MEAGPPGSAR PAEPGPCLSG QRGADHTASA SLQSVAGTEP GRHPQAVAAV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against UBA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1058 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBA1 antibody (ABIN504512)

Rogers, Olivier, Meyerholz, Dupuy: "Adaptive immunity does not strongly suppress spontaneous tumors in a Sleeping Beauty model of cancer." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 190, Issue 8, pp. 4393-9, (2013) (PubMed).

Target Details for UBA1

Target: UBA1 (Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1))

Alternative Name: UBA1

Background: UBA1 activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.
Alias Symbols: A1S9, A1S9T, A1ST, GXP1, MGC4781, UBA1A, UBE1, UBE1X, AMCX1, POC20, SMAX2
Protein Interaction Partner: USP5, UBE2D2, UBC, FUS, ISG15, UBTD2, HUWE1, UBE2L3, UBE2D1, SUMO2, CDC34, UBE2K, UBE2W, UBE2R2, UBE2J1, UBE2T, UBE2S, UBE2C, UBE2E3, UBE2H, UBE2G2, UBE2G1, UBE2E2, UBE2E1, UBE2D3, UBE2B, UBE2A, UBI4, MDM2, TRIM25, XPNPEP1, EZR, MC1R, IPO5, FH, CTPS1, ATI
Protein Size: 1058

Molecular Weight: 116 kDa

Gene ID: 7317

NCBI Accession: NM_003334, NP_003325

UniProt: P22314