RAD21 antibody (C-Term)

Catalog No. ABIN567792

This anti-RAD21 antibody is a Rat Monoclonal antibody detecting RAD21 in WB and IP. Suitable for Human, Mouse and Rat.

Quick Overview for RAD21 antibody (C-Term) (ABIN567792)

Target: RAD21 (RAD21 Homolog (RAD21))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: CM110-2C10

Product Details anti-RAD21 Antibody

Binding Specificity: AA 544-631, C-Term

Specificity: This antibody reacts with Cohesin (120 kDa) on Western blotting.

Purification: Protein-A Agarose Chromatography of hybridoma supernatant.

Immunogen: Recombinant C-terminal fragment (544-631 aa) of Scc1 subunit of Human Cohesin (hRad21).

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: RAD21

Background: Cohesin in eukaryotic cells is mediated by a multisubunit protein complex. Cohesin consists of four proteins: Smc1, Smc3, Scc1/Mcd1/hRad21, which is called kleisin and is the target of the protease separase, and Scc3/Irr1. Cohesin holds sister chromatids together until they separate into daughter cells during mitosis and also prevents splitting of chromosomes once bipolar attachments are made.Synonyms: Double-strand-break repair protein rad21 homolog, HR21, KIAA0078, NXP1, Nuclear matrix protein 1, SCC1 homolog

Gene ID: 5885

NCBI Accession: NP_006256

UniProt: O60216

Pathways: Positive Regulation of Endopeptidase Activity