Caspase 12 antibody (N-Term)

Catalog No. ABIN567796

This Rat Monoclonal antibody specifically detects Caspase 12 in WB, IF and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Caspase 12 antibody (N-Term) (ABIN567796)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Human, Mouse, Rat

Host: Rat

Clonality: Monoclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 14F4

Product Details anti-Caspase 12 Antibody

Binding Specificity: AA 95-318, N-Term

Specificity: This antibody reacts with Caspase-12 (~53 kDa) on Western blotting, Immunoprecipitation and Immunocytochemistry.

Purification: Protein-G Agarose Chromatography of hybridoma supernatant.

Immunogen: Recombinant Caspase-12 N-terminal fragment (aa 95-318).

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Caspase-12

Background: Caspase-12 is localized to the endoplasmic reticulum (ER), but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis and accumulation of excess proteins in ER, but not by membrane- or mitochondrial-targeted apoptotic signals. One well-known cause of ER stress in neurons is the accretion of amyloid-beta, the insoluble protein that forms plaques in the brains of patients with Alzheimer's disease. Caspase-12 mediates cytotoxicity induced by amyloid-beta and ER stress-induced apoptosis. Caspase-12 is ubiquitously expressed in tissues. Similar to other members of the Caspase family, Caspase-12 requires cleavage of the prodomain to activate its proapoptotic form.Synonyms: CASP-12, CASP12, Inactive caspase-12

Gene ID: 100506742

NCBI Accession: NP_001177945

UniProt: Q6UXS9

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response