DCTN3 antibody (Internal Region)

Catalog No. ABIN569634

Product Details anti-DCTN3 Antibody

Target: DCTN3 (Dctn3) (Dynactin 3 (p22) (Dctn3))

Binding Specificity: Internal Region

Reactivity: Human, Rat, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DCTN3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QVALGNISSK RE

Specificity: This antibody is expected to recognize both reported isoforms (NP_009165.1, NP_077324.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_009165.1 and NP_077324.1. Genename: DCTN3

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DCTN3

Target: DCTN3 (Dctn3) (Dynactin 3 (p22) (Dctn3))

Alternative Name: Dynactin Subunit 3 (Dctn3 Products)

Background: This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing of this gene generates 2 transcript variants. Transcript Variant: This variant contains an additional 97 nt within the 3' coding region, as compared to variant 1 resulting in a frame-shift. Isoform 2 encoded by this variant has a different, and 10 aa shorter C-terminus than isoform 1.Synonyms: DCTN22, DCTN3

Molecular Weight: 19.5kDa (NP_077324.1).

Gene ID: 11258

NCBI Accession: NP_009165

UniProt: O75935

Pathways: M Phase