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CD3 antibody

The Mouse Monoclonal anti-CD3 antibody has been validated for FACS, IHC and IHC (fro). It is suitable to detect CD3 in samples from Human and Monkey.
Catalog No. ABIN600629

Quick Overview for CD3 antibody (ABIN600629)

Target

See all CD3 Antibodies
CD3

Reactivity

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Human, Monkey

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This CD3 antibody is un-conjugated

Application

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Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone

UCHT1
  • Specificity

    Recognizes human CD3, a surface protein of peripheral blood T cells at ~19kD. Present on more than 95 % of circulating human peripheral T cells. Species cross-reactivity: cynomolgus monkey, chimpanzee.

    Purification

    Protein G purified

    Immunogen

    Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.

    Type of Immunogen: Cells

    Isotype

    IgG1
  • Application Notes

    Approved: Flo (1:100 - 1:200), IHC, IHC-Fr

    Usage: Suitable for use in Flow Cytometry and Immunohistochemistry (frozen). Flow Cytometry: 1:100-1:200, 10 μL labels 10^6 cells in 100 μL.

    Comment

    Target Species of Antibody: Human

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    Buffer contains: 0.09 % sodium azide

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeated freezing and thawing

    Storage

    4 °C,-20 °C

    Storage Comment

    May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.
  • Target

    CD3

    Alternative Name

    CD3

    Pathways

    TCR Signaling, Ubiquitin Proteasome Pathway
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