Sheep anti-Rabbit IgG (Heavy & Light Chain) Antibody (TRITC)

Catalog No. ABIN619446

Quick Overview for Sheep anti-Rabbit IgG (Heavy & Light Chain) Antibody (TRITC) (ABIN619446)

Target: IgG

Clonality: Polyclonal

Conjugate: TRITC

Application: Flow Cytometry (FACS), Immunomicroscopy (IM)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Sheep

Characteristics: Sheep anti-rabbit IgG (H&L) is a secondary antibody conjugated to TRITC, which binds to rabbit IgG (H&L) in immunological assays. Antibody is affinity purified using solid phase rabbit IgG (H&L). Tetramethylrhodamine-5-isothiocyanate (TRITC) has Amax = 550 nm, Emax = 570 nm.

Purification: Affinity purification

Immunogen: Purified rabbit IgG (H&L)

Application Details

Application Notes: This conjugate is suitable for immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2000 for most applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Prepare working dilution prior to use and then discard.
Product is photosensitive and should be protected from light.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: IgG

Target Type: Antibody