CYP21A2 antibody (AA 216-246)

Catalog No. ABIN652409

Product Details anti-CYP21A2 Antibody

Target: CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))

Binding Specificity: AA 216-246

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP21A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the Central region of human CYP21A2.

Clone: RB16954

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-CYP21A2 antibody (ABIN652409)

OShaughnessy, Monteiro, Bhattacharya, Fraser, Fowler: "Steroidogenic enzyme expression in the human fetal liver and potential role in the endocrinology of pregnancy." in: Molecular human reproduction, Vol. 19, Issue 3, pp. 177-87, (2013) (PubMed).

Target Details for CYP21A2

Target: CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))

Alternative Name: CYP21A2 (CYP21A2 Products)

Background: CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.

Molecular Weight: 55887

Gene ID: 1589

NCBI Accession: NP_000491, NP_001122062

UniProt: P08686

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process