CYP21A2 antibody (AA 314-363)

Catalog No. ABIN6753003

The Rabbit Polyclonal anti-CYP21A2 antibody is suitable to detect CYP21A2 in samples from Human. It has been validated for WB, IHC and IHC (p).

Quick Overview for CYP21A2 antibody (AA 314-363) (ABIN6753003)

Target: CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CYP21A2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CYP21A2 Antibody

Binding Specificity: AA 314-363

Specificity: Human CYP21A2 / 21-Hydroxylase

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa314-363 of human CYP21A2 (Q16874, NP_000491). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Marmoset (100%), Monkey, Panda, Dog, Cat, Bovine (92%), Pig (91%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CYP21A2

Target: CYP21A2 (Cytochrome P450, Family 21, Subfamily A, Polypeptide 2 (CYP21A2))

Alternative Name: CYP21A2

Background: Name/Gene ID: CYP21A2
Family: Cytochrome P450

Synonyms: CYP21A2, 21-OHase, CAH1, CYP21B, Cytochrome P450-C21, Cytochrome P450 21, Cytochrome P450 XXI, p450c21B, CA21H, CYP21, Cytochrome P-450c21, Cytochrome P450-C21B, Steroid 21-hydroxylase, Steroid 21-monooxygenase

Gene ID: 1589

NCBI Accession: NP_000491

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process