Leucyl/cystinyl Aminopeptidase (LNPEP) (N-Term), (AA 23-52) antibody

Details for Product No. ABIN654230
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Antigen
Synonyms IRAP, P-LAP, Vp165, CAP, PLAP, 2010309L07Rik, 4732490P18Rik, gp160, vp165, fb73h12, wu:fb73h12, zgc:66103
Epitope
N-Term, AA 23-52
(6), (2), (2), (1), (1)
Reactivity
Human
(16), (4), (2)
Host
Rabbit
(14), (3), (1)
Clonality (Clone)
Polyclonal ()
Application
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
(18), (13), (6), (5), (3), (2), (2), (2), (1)
Pubmed 2 references available
Catalog no. ABIN654230
Quantity 400 µL
Price
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Immunogen This LNPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-52 AA from the N-terminal region of human LNPEP.
Clone RB27638
Isotype Ig
Specificity This LNPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human LNPEP.
Predicted Reactivity Mouse (Murine),Rat (Rattus)
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name LNPEP
Background This gene encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Synonyms: Leucyl-cystinyl aminopeptidase,LNPEP,OTASE
Molecular Weight 117349 DA
Gene ID 4012
UniProt NP_005566.2, NP_787116.2, Q9UIQ6
Research Area Phospho-specific antibodies, Cell Signaling, Cell Structure, Protein Modifications, Metabolism
Application Notes WB = 1:1000, IHC = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Leucyl/cystinyl Aminopeptidase (LNPEP) (N-Term), (AA 23-52) antibody LNPEP Antibody (N-Term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the LNPEP antibody detected the LNPEP protein (arrow).
anti-Leucyl/cystinyl Aminopeptidase (LNPEP) (N-Term), (AA 23-52) antibody (2) LNPEP antibody (N-Term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LNPEP antibody (N-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
anti-Leucyl/cystinyl Aminopeptidase (LNPEP) (N-Term), (AA 23-52) antibody (3) LNPEP Antibody (N-Term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Background publications Saveanu, Carroll, Weimershaus et al.: "IRAP identifies an endosomal compartment required for MHC class I cross-presentation." in: Science (New York, N.Y.), Vol. 325, Issue 5937, pp. 213-7, 2009 (PubMed).

Georgiadou, Hearn, Evnouchidou et al.: "Placental leucine aminopeptidase efficiently generates mature antigenic peptides in vitro but in patterns distinct from endoplasmic reticulum aminopeptidase 1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 3, pp. 1584-92, 2010 (PubMed).

Hosts (14), (3), (1)
Reactivities (16), (4), (2)
Applications (18), (13), (6), (5), (3), (2), (2), (2), (1)
Epitopes (6), (2), (2), (1), (1)
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Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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