BCKDHB antibody (N-Term)

Catalog No. ABIN654629

The Rabbit Polyclonal anti-BCKDHB antibody has been validated for WB, FACS, IF and IHC (p). It is suitable to detect BCKDHB in samples from Human.

Quick Overview for BCKDHB antibody (N-Term) (ABIN654629)

Target: BCKDHB (Branched Chain Keto Acid Dehydrogenase E1, beta Polypeptide (BCKDHB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCKDHB antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB20925

Product Details anti-BCKDHB Antibody

Binding Specificity: AA 41-70, N-Term

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This BCKDHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 41-70 amino acids from the N-terminal region of human BCKDHB.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:25. WB: 1:1000. WB: 1:1000. IHC-P-Leica: 1:500. IHC-P-Leica: 1:500. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

Target Details for BCKDHB

Target: BCKDHB (Branched Chain Keto Acid Dehydrogenase E1, beta Polypeptide (BCKDHB))

Alternative Name: BCKDHB

Background: Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria, and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit, and mutations therein have been associated with maple syrup urine disease (MSUD), type 1B, a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation, and feeding problems.

Molecular Weight: 43122

Gene ID: 594

NCBI Accession: NP_000047, NP_898871

UniProt: P21953