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Splicing Factor 3b, Subunit 1, 155kDa (SF3B1) (AA 374-402), (N-Term) antibody

Details for Product No. ABIN657676, Supplier: Login to see
Antigen
  • SF3B1
  • wu:fb99f09
  • 155kDa
  • 2810001M05Rik
  • AA409119
  • Prp10
  • SAP155
  • SF3b155
  • TA-8
  • Targ4
  • Sap155
  • hsh155
  • prp10
  • prpf10
  • sap155
  • Hsh155
  • MDS
  • PRP10
  • PRPF10
Epitope
AA 374-402, N-Term
30
15
2
2
2
2
1
1
1
1
Reactivity
Human
63
38
30
12
4
4
4
3
1
Host
Rabbit
60
4
Clonality (Clone)
Polyclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
48
23
21
11
7
3
3
1
Supplier
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Immunogen This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 374-402 AA from the N-terminal region of human SF3B1.
Clone RB33758
Isotype Ig
Specificity This SF3B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 373-402 amino acids from the N-terminal region of human SF3B1.
Predicted Reactivity Mouse (Murine),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Alternative Name SF3B1 (SF3B1 Antibody Abstract)
Background This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
Synonyms: Splicing factor 3B subunit 1,SF3B1,SAP155
Molecular Weight 145830 DA
Gene ID 23451
NCBI Accession NP_036565
UniProt O75533
Research Area Signaling, Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Pathways
Application Notes WB = 1:1000, IHC (p) = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 0.37 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment SF3B1 Antibody (N-term) can be refrigerated at 2-8 °C for up to 6 months. For long term storage, place the at -20 °C.
Expiry Date 6 months
Supplier Images
Western Blotting (WB) image for anti-Splicing Factor 3b, Subunit 1, 155kDa (SF3B1) (AA 374-402), (N-Term) antibody (ABIN657676) SF3B1 Antibody (N-term) (ABIN657676) western blot analysis in CEM cell line lysates (...
Background publications Corsini, Hothorn, Stier et al.: "Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 630-9, 2008 (PubMed).

Tanuma, Kim, Beullens et al.: "Nuclear inhibitor of protein phosphatase-1 (NIPP1) directs protein phosphatase-1 (PP1) to dephosphorylate the U2 small nuclear ribonucleoprotein particle (snRNP) component, spliceosome-associated protein 155 (Sap155)." in: The Journal of biological chemistry, Vol. 283, Issue 51, pp. 35805-14, 2008 (PubMed).

Pessa, Will, Meng et al.: "Minor spliceosome components are predominantly localized in the nucleus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 25, pp. 8655-60, 2008 (PubMed).