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SF3B1 antibody (AA 98-198)
SF3B1
Reactivity: Human, Mouse
WB, IF, IP
Host: Mouse
Monoclonal
16
unconjugated
Product Details anti-SF3B1 Antibody
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Target
See all SF3B1 Antibodies
SF3B1
(Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))
Binding Specificity
All epitopes for SF3B1 antibodies
AA 98-198
Reactivity
All reactivities for SF3B1 antibodies
Human, Mouse
Host
All hosts for SF3B1 antibodies
Mouse
Clonality
All clonalities for SF3B1 antibodies
Monoclonal
Conjugate
All conjugates for SF3B1 antibodies
This SF3B1 antibody is un-conjugated
Application
All applications for SF3B1 antibodies
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
Specificity
This antibody reacts with Sap155.
Cross-Reactivity (Details)
Species reactivity (tested):Human, Mouse
Purification
Protein A agarose
Immunogen
Recombinant GST-Sap155 (98 a.a.- 198 a.a.)
Clone
16
Isotype
IgG2b
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Discover our top product SF3B1 Primary Antibody
Alternatives
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anti-Splicing Factor 3b, Subunit 1, 155kDa (SF3B1) (N-Term) antibody
SF3B1
Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Goat, Rabbit, Zebrafish (Danio rerio)
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
anti-Splicing Factor 3b, Subunit 1, 155kDa (SF3B1) (Middle Region) antibody
SF3B1
Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Goat, Rabbit, Zebrafish (Danio rerio)
WB, IHC
Host: Rabbit
Polyclonal
unconjugated
Application Details
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Application Notes
Optimal working dilution should be determined by the investigator.
Restrictions
For Research Use only
Handling
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Concentration
1.0 mg/mL
Buffer
PBS containing 50 % glycerol, pH 7.2. Contains no preservatives.
Preservative
Without preservative
Handling Advice
Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Upon receipt, store (in aliqouts) at -20 °C.
References for anti-SF3B1 antibody (ABIN1449241)
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Salas-Armenteros, Barroso, Rondón, Pérez, Andújar, Luna, Aguilera : "Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability. " in: Cell reports , Vol. 28 , Issue 6 , pp. 1551-1563.e7 , (2020 ) (PubMed ).
Target Details for SF3B1
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Target
SF3B1
(Splicing Factor 3b, Subunit 1, 155kDa (SF3B1))
Alternative Name
SF3B1 / SAP155 (SF3B1 Products )
Synonyms
Cus1 antibody, SAP145 antibody, SF3B145 antibody, SF3b1 antibody, SF3b150 antibody, Hsh155 antibody, MDS antibody, PRP10 antibody, PRPF10 antibody, SAP155 antibody, SF3b155 antibody, 155kDa antibody, 2810001M05Rik antibody, AA409119 antibody, Prp10 antibody, TA-8 antibody, Targ4 antibody, hsh155 antibody, prp10 antibody, prpf10 antibody, sap155 antibody, SF3B1 antibody, Sap155 antibody, wu:fb99f09 antibody, splicing factor 3b subunit 2 antibody, splicing factor 3b subunit 1 antibody, splicing factor 3b, subunit 1 antibody, splicing factor 3b subunit 1 S homeolog antibody, SF3B2 antibody, SF3B1 antibody, Sf3b1 antibody, sf3b1.S antibody, sf3b1 antibody
Background
SF3 is a U2 snRNP-associated protein complex essential for spliceosome assembly and splicing catalysis of the majo r spliceosome. SF3 contains the Spliceosome-Associated Proteins, Sap 49, 130, 145, and 155. Sap155/Sf3b1 is an essential subunit of the U2 snRNP for mRNA splicing and has also been identified in the minor (U12-dependent) sp liceosome. Sap155 interacts with the mammalian PcG (Polycomb group) proteins, Mel18 and Ring1B by the yeast two hybrid system. Sap155 contains numerous Cdk consensus phosphorylation sites in its N terminus and is phosphorylated prior to catal ytic step II of the splicing pathway. Sap155 serves as a substrate for cyclin E-cdk2 in vitro , suggesting that pre-mRNA splicing may be linked to the cell cycle machiner y in mammalian cells.Synonyms: Pre-mRNA-splicing factor SF3b 155 kDa subunit, SAP 155, SAP-155, SF3b155, Spliceosome-associated protein 155, Splicing factor 3B subunit 1
Molecular Weight
155 kDa
Gene ID
23451
NCBI Accession
NP_036565
UniProt
O75533
Pathways
Chromatin Binding
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