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NMDA Receptor 2A (pTyr1325) antibody

Details for Product No. ABIN784606, Supplier: Log in to see
Mouse (Murine), Rat (Rattus)
Western Blotting (WB)
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Immunogen Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDA NR2A.
Isotype IgG
Specificity Specific for the ~180k NMDAR NR2A-subunit protein phosphorylated at Tyr1325 in Western blots. Immunolabeling is completely blocked by blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Cross-Reactivity (Details) Species reactivity (expected):Bovine and CanineSpecies reactivity (tested):Mouse and Rat.
Purification Affinity Chromatography.
Background The ion channels activated by glutamate that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). Channels with physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits (Ishii et al., 1993). Recently, phosphorylation of Tyrosine 1325 of the NR2A subunit has been shown to be increased in human brain tissue sections from HIV-infected individuals with encephalitis (King et al., 2010). In addition, Tyr1325 phosphorylation has been linked with depression-related behavior (Taniguchi et al., 2009).Synonyms: GRIN2A, Glutamate [NMDA] Receptor subunit epsilon-1, NMDAR2A, NR2A
Gene ID 24409
NCBI Accession NP_036705
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Buffer 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-NMDA Receptor 2A (pTyr1325) antibody (ABIN784606) Western blot of Rat hippocampal lysate showing specific immunolabeling of the ~180k N...
Background publications King, Eugenin, Hazleton, Morgello, Berman: "Mechanisms of HIV-tat-induced phosphorylation of N-methyl-D-aspartate receptor subunit 2A in human primary neurons: implications for neuroAIDS pathogenesis." in: The American journal of pathology, Vol. 176, Issue 6, pp. 2819-30, 2010 (PubMed).

Taniguchi, Nakazawa, Tanimura, Kiyama, Tezuka, Watabe, Katayama, Yokoyama, Inoue, Izumi-Nakaseko, Kakuta, Sudo, Iwakura, Umemori, Inoue, Murphy, Hashimoto, Kano, Manabe, Yamamoto: "Involvement of NMDAR2A tyrosine phosphorylation in depression-related behaviour." in: The EMBO journal, Vol. 28, Issue 23, pp. 3717-29, 2009 (PubMed).

Alvestad, Grosshans, Coultrap, Nakazawa, Yamamoto, Browning: "Tyrosine dephosphorylation and ethanol inhibition of N-Methyl-D-aspartate receptor function." in: The Journal of biological chemistry, Vol. 278, Issue 13, pp. 11020-5, 2003 (PubMed).

Wenthold, Prybylowski, Standley, Sans, Petralia: "Trafficking of NMDA receptors." in: Annual review of pharmacology and toxicology, Vol. 43, pp. 335-58, 2003 (PubMed).

Carroll, Zukin: "NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity." in: Trends in neurosciences, Vol. 25, Issue 11, pp. 571-7, 2002 (PubMed).

Grosshans, Clayton, Coultrap, Browning: "LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1." in: Nature neuroscience, Vol. 5, Issue 1, pp. 27-33, 2001 (PubMed).

Snell, Nunley, Lickteig, Browning, Tabakoff, Hoffman: "Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion." in: Brain research. Molecular brain research, Vol. 40, Issue 1, pp. 71-8, 1997 (PubMed).

Ishii, Moriyoshi, Sugihara, Sakurada, Kadotani, Yokoi, Akazawa, Shigemoto, Mizuno, Masu: "Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits." in: The Journal of biological chemistry, Vol. 268, Issue 4, pp. 2836-43, 1993 (PubMed).

Lovinger, White, Weight: "Ethanol inhibits NMDA-activated ion current in hippocampal neurons." in: Science (New York, N.Y.), Vol. 243, Issue 4899, pp. 1721-4, 1989 (PubMed).