NMDAR2A antibody (C-Term)

Catalog No. ABIN361396

Product Details anti-NMDAR2A Antibody

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Binding Specificity: C-Term

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMDAR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Specific for the ~180k NR2A subunit of the NMDA receptor. Recognizes human, mouse and rat forms of the NR2A subunit of NMDAR. No reactivity towards the NR2B and NR2C subunits. Immunolabeling is blocked by pre-adsorption of antibody with the fusion protein used to generate the antibody.

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Fusion protein from the C-terminal region of the NR2A subunit

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IHC (frozen sections, unpublished observations): 1:1000 to 1:2000 IP: 3 µl per 200 µg lysate Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized

Storage: -20 °C

References for anti-NMDAR2A antibody (ABIN361396)

Morimura, Yasuda, Yamaguchi, Katayama, Hatayama, Tomioka, Odagawa, Kamiya, Iwayama, Maekawa, Nakamura, Matsuzaki, Tsujii, Yamada, Yoshikawa, Aruga: "Autism-like behaviours and enhanced memory formation and synaptic plasticity in Lrfn2/SALM1-deficient mice." in: Nature communications, Vol. 8, pp. 15800, (2018) (PubMed).

Target Details for NMDAR2A

Target: NMDAR2A (GRIN2A) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2a (GRIN2A))

Alternative Name: GRIN2A (GRIN2A Products)

Synonyms: NMDAR2A antibody, nr2a antibody, GRIN2A antibody, EPND antibody, GluN2A antibody, NR2A antibody, glutamate ionotropic receptor NMDA type subunit 2A antibody, glutamate receptor, ionotropic, N-methyl D-aspartate 2A L homeolog antibody, glutamate receptor ionotropic, NMDA 2A antibody, glutamate receptor, ionotropic, NMDA2A (epsilon 1) antibody, GRIN2A antibody, grin2a.L antibody, LOC100438553 antibody, Grin2a antibody

Background: The ion channels activated by glutamate are typically divided into two classes. Glutamate receptors that are activated by kainate and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as kainate/AMPA receptors (K/AMPAR). Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002, Wenthold et al., 2003, Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989, Alvestad et al., 2003, Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. Anti-NMDA Receptor, NR2A Subunit Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~180k NR2A subunit of the NMDA receptor.

Molecular Weight: '180 kDa

Gene ID: 24409

UniProt: Q00959

Pathways: Synaptic Membrane, Regulation of long-term Neuronal Synaptic Plasticity