TAR DNA Binding Protein (TARDBP) (C-Term), (Cleavage Site) antibody

Details for Product No. ABIN784609
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Antigen
Synonyms TARDBP, TDP43, DKFZp459I2127, tardbp, ALS10, TDP-43, 1190002A23Rik, C85084, Tdp43, wu:fb77f02, wu:fc52g10
Epitope
C-Term, Cleavage Site
(22), (20), (14), (13), (7), (7), (7), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus)
(119), (59), (49), (23), (16), (8), (7), (6), (5), (3), (1), (1)
Host
Rabbit
(91), (29), (5), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(108), (49), (30), (24), (24), (23), (10), (6), (2), (1), (1), (1)
Pubmed 3 references available
Quantity 0.1 mL
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Catalog No. ABIN784609
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Immunogen Synthetic peptide C-terminal to the caspase-cleavage site (between D219 and V220) of Human TDP-43.
Isotype IgG
Specificity Specific for the ~ 43kDa TDP-43 protein in Western blots of Rat brain lysate. Expected molecular weight is dependent upon the TDP-43 species present in sample (full-length vs. truncated TDP-43). Under non-denaturing conditions (for example, by IHC), this antibody detects TDP-43 inclusions in Human brain tissue with TDP-43 proteinopathy, but does not detect full-length nuclear TDP-43. Under denaturing conditions (for example, by Western Blot analysis) this antibody detects the C-terminus of full-length and truncated Human TDP-43.
Purification Affinity Chromatography.
Alternative Name TARDBP
Background TDP43 (Tar DNA Binding 43, TARDBP) was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (Ou et al.,1995). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (Neumann et al., 2006). The protein is present in these inclusions in partially degraded, hyperphosphorylated and ubiquitinated forms.
Alternate names: TAR DNA-binding protein 43, TDP-43, TDP43
Gene ID 23435
NCBI Accession NP_031401
Research Area Chromatin and Nuclear Signaling, Transcription Factors, DNA/RNA, Hormones, Cytokines
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 30% Glycerol.
Handling Advice Avoid repeated freezing and thawing.
Storage -80 °C
Storage Comment Store the antibody undiluted (in aliquots) at -80°C.
Expiry Date 6 months
Background publications Ou, Wu, Harrich et al.: "Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs." in: Journal of virology, Vol. 69, Issue 6, pp. 3584-96, 1995 (PubMed).

Neumann, Sampathu, Kwong et al.: "Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis." in: Science (New York, N.Y.), Vol. 314, Issue 5796, pp. 130-3, 2006 (PubMed).

Forman, Trojanowski, Lee: "TDP-43: a novel neurodegenerative proteinopathy." in: Current opinion in neurobiology, Vol. 17, Issue 5, pp. 548-55, 2008 (PubMed).

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