CD103 antibody (FITC)

Catalog No. ABIN810081

This anti-CD103 antibody is a Mouse Monoclonal antibody detecting CD103 in FACS. Suitable for Human and Non-Human Primate. This Primary Antibody has been cited in 9+ publications.

Quick Overview for CD103 antibody (FITC) (ABIN810081)

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD103 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: Ber-ACT8

Product Details anti-CD103 Antibody (FITC)

Purpose: Anti-Hu CD103 FITC

Specificity: The mouse monoclonal antibody Ber-ACT8 recognizes an extracellular epitope of CD103 (alpha E integrin), a type I transmembrane glycoprotein primarily found on intestinal intraepithelial lymphocytes.

Cross-Reactivity (Details): Human, Non-Human Primates

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: HTLV-1 induced human T cell line MAPS16

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD103 antibody (FITC) (ABIN810081)

Zhang, Bertucci, Ramsey-Goldman, Burt, Datta et al.: "Regulatory T cell (Treg) subsets return in patients with refractory lupus following stem cell transplantation, and TGF-beta-producing CD8+ Treg cells are associated with immunological remission of ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 10, pp. 6346-58, (2009) (PubMed).

Jaensson, Uronen-Hansson, Pabst, Eksteen, Tian, Coombes, Berg, Davidsson, Powrie, Johansson-Lindbom, Agace: "Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans." in: The Journal of experimental medicine, Vol. 205, Issue 9, pp. 2139-49, (2008) (PubMed).

Cardoso, Garlet, Moreira, Júnior, Rossi, Silva: "Characterization of CD4+CD25+ natural regulatory T cells in the inflammatory infiltrate of human chronic periodontitis." in: Journal of leukocyte biology, Vol. 84, Issue 1, pp. 311-8, (2008) (PubMed).

Kost, Holden, Mann: "Marginal zone B-cell lymphoma: a retrospective immunophenotypic analysis." in: Cytometry. Part B, Clinical cytometry, Vol. 74, Issue 5, pp. 282-6, (2008) (PubMed).

Sigmundsdottir, Johnston, Gudjonsson, Bjarnason, Valdimarsson: "Methotrexate markedly reduces the expression of vascular E-selectin, cutaneous lymphocyte-associated antigen and the numbers of mononuclear leucocytes in psoriatic skin." in: Experimental dermatology, Vol. 13, Issue 7, pp. 426-34, (2004) (PubMed).

León, Roy: "Isolation of human small bowel intraepithelial lymphocytes by annexin V-coated magnetic beads." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 84, Issue 6, pp. 804-9, (2004) (PubMed).

Brandes, Willimann, Lang, Nam, Jin, Brenner, Morita, Moser: "Flexible migration program regulates gamma delta T-cell involvement in humoral immunity." in: Blood, Vol. 102, Issue 10, pp. 3693-701, (2003) (PubMed).

Poles, Barsoum, Yu, Yu, Sun, Daly, He, Mehandru, Talal, Markowitz, Hurley, Ho, Zhang: "Human immunodeficiency virus type 1 induces persistent changes in mucosal and blood gammadelta T cells despite suppressive therapy." in: Journal of virology, Vol. 77, Issue 19, pp. 10456-67, (2003) (PubMed).

French, Cresswell, Wong, Seymour, Charnley, Kirby: "T cell adhesion and cytolysis of pancreatic cancer cells: a role for E-cadherin in immunotherapy?" in: British journal of cancer, Vol. 87, Issue 9, pp. 1034-41, (2002) (PubMed).

Target Details for CD103

Target: CD103 (ITGAE) (Integrin, alpha E (Antigen CD103, Human Mucosal Lymphocyte Antigen 1, alpha Polypeptide) (ITGAE))

Alternative Name: CD103

Background: Integrin subunit alpha E,CD103 / integrin alphaE is an integrin subunit that is expressed on intraepithelial lymphocytes, epithelial dendritic cells, lamina propria-derived dendritic cells, a subpopulation of lamina propria T cells, a small subset of peripheral lymphocytes, namely T reg cells, and on activated and TGF-beta stimulated lymphocytes. CD103 is in mature form cleaved into two disulfide-linked chains (C-terminal 150 kDa chain and N-terminal 25 kDa chain). It heterodimerizes with integrin beta7 subunit to form alphaE/beta7 integrin (mucosal lymphocyte 1 antigen), which through binding E-cadherin mediates homing of lymphocytes to the intestinal epithelium, and, in addition to the role in adhesion, may serve as an accessory molecule for intraepithelial lymphocyte activation.,Integrin alpha E, ITGAE

Gene ID: 3682

UniProt: P38570