CDK1 antibody

Catalog No. ABIN931491

Product Details anti-CDK1 Antibody

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Immunogen: Cdc2 antibody was raised in Mouse using a purified recombinant fragment of Cdc2 expressed in E. coli as the immunogen.

Clone: 8C5A7F10

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:10,000, FC: 1:200-1:400, WB: 1:500-1:2000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term storage. Store at -20 °C for long term storage.

Target Details for CDK1

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Alternative Name: Cdc2 (CDK1 Products)

Background: Cdc2 plays a key role in the control of the eukaryotic cell cycle, where it is required for entry into S-phase and mitosis. Cdc2 exists as a complex with both cyclin A and cyclin B. The best characterized of these associations is the Cdc2 p34 cyclin B complex, which is required for the G2 to M phase transition. Activation of Cdc2 is controlled at several steps including cyclin binding and phosphorylation of threonine 161. However, the critical regulatory step in activating Cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Tyr14. Phosphorylation at Tyr15 and inhibition of Cdc2 is carried out by WEE1 and MIK protein kinases while Tyr15 dephosphorylation and activation of Cdc2 is carried out by the Cdc25 phosphatase. The isoform Cdc2deltaT is found in breast cancer tissues. Furthermore, Cdc2/Cdk1 is a key mediator of neuronal cell death in brain development and degeneration. Synonyms: Monoclonal Cdc2 antibody, Anti-Cdc2 antibody, Cyclin dependent kinase 1 antibody, Cdk1 antibody, Cell division control protein 2 homolog antibody.

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA