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GRP94 antibody (Middle Region)

Name: GRP94 antibody (Middle Region)
SKU: ABIN952082
Price: 768 USD
Availability: InStock

HSP90B1 Reactivity: Human WB, FACS, IHC (p), EIA Host: Rabbit Polyclonal unconjugated

Western blot analysis of Endoplasmin / HSP90B1 / TRA1 Antibody (Center) in K562, A549, Hela, NCI-H460, NCI-H292, ZR-75-1 cell line lysates (35ug/lane). (GRP94 antibody (Middle Region))

Flow cytometric analysis of A549 cells using Endoplasmin / HSP90B1 / TRA1 Antibody (Center) Cat. (GRP94 antibody (Middle Region))

FACS

Immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue reacted with Endoplasmin / HSP90B1 / TRA1 Antibody (Center) followed which was peroxidase conjugated to the secondary antibody and followed by DAB staining. (GRP94 antibody (Middle Region))

IHC (p)

3 images

Catalog No. ABIN952082

Datasheet as PDF

Target See all GRP94 (HSP90B1) Antibodies

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
Binding Specificity
17

16

16

7

7

4

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1
AA 467-495, Middle Region
Reactivity
126

92

57

38

33

29

28

28

26

26

25

24

24

15

10

2

2

1

1
Human
Host
91

38

20

2

1
Rabbit
Compatible Secondaries
Clonality
92

60
Polyclonal
Conjugate
65

10

9

8

7

6

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GRP94 antibody is un-conjugated
Application
133

74

62

54

43

37

33

16

13

13

13

11

4

3

2

2
Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity

This antibody recognizes Human Endoplasmin / HSP90B1 / TRA1 (Center).

Purification

Protein A column, followed by peptide affinity purification

Immunogen

KLH conjugated synthetic peptide between 467-495 amino acids from the Central region of human HSP90B1

Isotype

Ig Fraction

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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibody
HSP90B1 Reactivity: Human, Mouse WB, IF, IP, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

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HSP90B1 Reactivity: Human WB, IF, IHC (p), EIA Host: Mouse Monoclonal 2H3 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody
HSP90B1 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 16-43), (N-Term) antibody
HSP90B1 Reactivity: Human, Mouse, Hamster WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB25612 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis WB, IHC, IF, FACS, StM Host: Rat Monoclonal 9G10-F8-2 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis IHC, FACS, StM Host: Rabbit Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibody
HSP90B1 Reactivity: Human, Mouse, Rat WB, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Application Notes

Optimal working dilution should be determined by the investigator.

Restrictions

For Research Use only
Format

Liquid

Concentration

0.25 mg/mL

Buffer

PBS containing 0.09 % (W/V) Sodium Azide as preservative

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C/-20 °C

Storage Comment

Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Target

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name

Endoplasmin / HSP90B1 / TRA1 (HSP90B1 Products)

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1, MIM 140571) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1

Molecular Weight

92469 Da

Gene ID

7184

NCBI Accession

NP_003290

Pathways

Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades