Mitogen-Activated Protein Kinase Kinase Kinase 4 (MAP3K4) (AA 1065-1097), (Middle Region) antibody

Details for Product No. ABIN953298
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Antigen
Synonyms MAPKKK4, MEKK4, MTK1, PRO0412, D17Rp17, D17Rp17e, MEKK 4, Mek4b, Mekk4, RP17, Rp17a, Tas, mKIAA0213
Epitope
AA 1065-1097, Middle Region
(15), (7), (6), (1), (1), (1)
Reactivity
Human
(52), (27), (14), (12), (12), (12), (12)
Host
Rabbit
(34), (18)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(31), (30), (23), (10), (9), (2), (1), (1), (1)
Pubmed 5 references available
Quantity 0.4 mL
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Catalog No. ABIN953298
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Immunogen KLH conjugated synthetic peptide between 1065-1097 amino acids from the Central region of human MEKK4 Genename: MAP3K4
Specificity This antibody reacts to MEKK4.
Purification Protein A column, followed by peptide affinity purification
Alternative Name MAP3K4
Background The central core of each mitogen-activated protein kinase (MAPK) pathway is a conserved cascade of 3 protein kinases: an activated MAPK kinase kinase (MAPKKK) phosphorylates and activates a specific MAPK kinase (MAPKK), which then activates a specific MAPK. While the ERK MAPKs are activated by mitogenic stimulation, the CSBP2 and JNK MAPKs are activated by environmental stresses such as osmotic shock, UV irradiation, wound stress, and inflammatory factors. This gene encodes a MAPKKK, the MEKK4 protein, also called MTK1. This protein contains a protein kinase catalytic domain at the C terminus. The N- terminal nonkinase domain may contain a regulatory domain. Expression of MEKK4 in mammalian cells activated the CSBP2 and JNK MAPK pathways, but not the ERK pathway. In vitro kinase studies indicated that recombinant MEKK4 can specifically phosphorylate and activate PRKMK6 and SERK1, MAPKKs that activate CSBP2 and JNK, respectively but cannot phosphorylate PRKMK1, an MAPKK that activates ERKs. MEKK4 is a major mediator of environmental stresses that activate the CSBP2 MAPK pathway, and a minor mediator of the JNK pathway. Two alternatively spliced transcripts encoding distinct isoforms have been described.
Alternate names: MAP three kinase 1, MAPK/ERK kinase kinase 4, MAPKKK4, MEKK4, MTK1, Mitogen- activated protein kinase kinase kinase 4
Gene ID 4216
NCBI Accession NP_005913
Restrictions For Research Use only
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS containing 0.09% (w/v) Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Derbyshire, Halfter, Heimark et al.: "Angiotensin II stimulated transcription of cyclooxygenase II is regulated by a novel kinase cascade involving Pyk2, MEKK4 and annexin II." in: Molecular and cellular biochemistry, Vol. 271, Issue 1-2, pp. 77-90, 2005 (PubMed).

Abell, Johnson: "MEKK4 is an effector of the embryonic TRAF4 for JNK activation." in: The Journal of biological chemistry, Vol. 280, Issue 43, pp. 35793-6, 2005 (PubMed).

Aissouni, Zapart, Iovanna et al.: "CIN85 regulates the ability of MEKK4 to activate the p38 MAP kinase pathway." in: Biochemical and biophysical research communications, Vol. 338, Issue 2, pp. 808-14, 2005 (PubMed).

Whitmarsh, Davis: "Role of mitogen-activated protein kinase kinase 4 in cancer." in: Oncogene, Vol. 26, Issue 22, pp. 3172-84, 2007 (PubMed).

Grucza, Johnson, Krueger et al.: "Incorporating age at onset of smoking into genetic models for nicotine dependence: evidence for interaction with multiple genes." in: Addiction biology, Vol. 15, Issue 3, pp. 346-57, 2010 (PubMed).

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