RASGRP2 antibody (N-Term)

Catalog No. ABIN954463

Product Details anti-RASGRP2 Antibody

Target: RASGRP2 (RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2))

Binding Specificity: AA 14-42, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RASGRP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody recognizes Human RASGRP2 (N-term).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Bovine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A

Immunogen: KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of Human RASGRP2. Genename: RASGRP2

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-RASGRP2 antibody (ABIN954463)

Lima, Parreira, Devuyst, Caplanusi, Nkuli, Marien, Van Der Smissen, Alves, Verroust, Christensen, Terzi, Matter, Balda, Pierreux, Courtoy: "ZONAB promotes proliferation and represses differentiation of proximal tubule epithelial cells." in: Journal of the American Society of Nephrology : JASN, Vol. 21, Issue 3, pp. 478-88, (2010) (PubMed).

Katagiri, Shimonaka, Kinashi: "Rap1-mediated lymphocyte function-associated antigen-1 activation by the T cell antigen receptor is dependent on phospholipase C-gamma1." in: The Journal of biological chemistry, Vol. 279, Issue 12, pp. 11875-81, (2004) (PubMed).

Eto, Murphy, Kerrigan, Bertoni, Stuhlmann, Nakano, Leavitt, Shattil: "Megakaryocytes derived from embryonic stem cells implicate CalDAG-GEFI in integrin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 20, pp. 12819-24, (2002) (PubMed).

Guo, Kumahara, Saffen: "A CalDAG-GEFI/Rap1/B-Raf cassette couples M(1) muscarinic acetylcholine receptors to the activation of ERK1/2." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25568-81, (2001) (PubMed).

Toki, Kawasaki, Tashiro, Housman, Graybiel: "Guanine nucleotide exchange factors CalDAG-GEFI and CalDAG-GEFII are colocalized in striatal projection neurons." in: The Journal of comparative neurology, Vol. 437, Issue 4, pp. 398-407, (2001) (PubMed).

Target Details for RASGRP2

Target: RASGRP2 (RAS Guanyl Releasing Protein 2 (Calcium and DAG-Regulated) (RASGRP2))

Alternative Name: RASGRP2 (RASGRP2 Products)

Background: The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a C-terminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene.Synonyms: CDC25L, CalDAG-GEFI, Calcium and DAG-regulated guanine nucleotide exchange factor I, Cdc25-like protein, MCG7, RAS guanyl-releasing protein 2

Molecular Weight: 75kd

Gene ID: 10235

NCBI Accession: NP_001092140

Pathways: TCR Signaling