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Forkhead Box O1 (FOXO1) antibody

Antigen

Forkhead Box O1 (FOXO1)
Synonyms FKH1, FKHR, FOXO1A, Afxh, Fkhr1, Foxo1a, AI876417, Fkhr, FOXO1, fkh1, fkhr, foxo1a, xFoxO1, MGC79506
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN966149
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name FKHR
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human FKHR around the phosphorylation site of serine 319.
Specificity FKHR (Ab-319) antibody detects endogenous levels of total FKHR protein.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Guo, Rena, Cichy et al.: "Phosphorylation of serine 256 by protein kinase B disrupts transactivation by FKHR and mediates effects of insulin on insulin-like growth factor-binding protein-1 promoter activity through a conserved insulin response sequence." in: The Journal of biological chemistry, Vol. 274, Issue 24, pp. 17184-92, 1999 (PubMed).

Rena, Prescott, Guo et al.: "Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting." in: The Biochemical journal, Vol. 354, Issue Pt 3, pp. 605-12, 2001 (PubMed).

Rena, Woods, Prescott et al.: "Two novel phosphorylation sites on FKHR that are critical for its nuclear exclusion." in: The EMBO journal, Vol. 21, Issue 9, pp. 2263-71, 2002 (PubMed).

Zhao, Gan, Pan et al.: "Multiple elements regulate nuclear/cytoplasmic shuttling of FOXO1: characterization of phosphorylation- and 14-3-3-dependent and -independent mechanisms." in: The Biochemical journal, Vol. 378, Issue Pt 3, pp. 839-49, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Forkhead Box O1 (FOXO1)", type "Antibodies"
Hosts Rabbit (163), Mouse (22), Goat (6), Sheep (1)
Reactivities Human (191), Mouse (Murine) (121), Rat (Rattus) (116), Cow (Bovine) (78), Dog (Canine) (78), Pig (Porcine) (76), Chicken (55), Horse (Equine) (54), Rabbit (18), Monkey (2), Xenopus laevis (2), Zebrafish (2)
Applications Western Blotting (WB) (120), Immunofluorescence (IF) (84), Immunohistochemistry (IHC) (45), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (45), ELISA (43), Immunocytochemistry (ICC) (17), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (12), Immunoprecipitation (IP) (6), Immunoelectron Microscopy (IEM) (4), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), Biotin (4), Cy3 (4), Cy5 (4), Cy5.5 (4), Cy7 (4), FITC (4), Gold (4), HRP (4), PE (4), PE,Cy3 (3), PE,Cy5 (3), PE,Cy5.5 (3), PE,Cy7 (3), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes pSer256 (34), pSer319 (32), AA 242-655 (8), N-Term (7), Internal Region (6), C-Term (4), Ser256 (3), AA 254-258 (2), Ser319 (2), pSer329 (2), AA 250-300 (1), AA 353-655 (1), Center (1), Center,Asp469 (1)