FOXO1 antibody

Catalog No. ABIN966149

Product Details anti-FOXO1 Antibody

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: FKHR (Ab-319) antibody detects endogenous levels of total FKHR protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human FKHR around the phosphorylation site of serine 319.

Application Details

Application Notes: WB: 1:500~1:1000
IHC: 1:50~1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-FOXO1 antibody (ABIN966149)

Zhao, Gan, Pan, Kan, Majeski, Adam, Unterman: "Multiple elements regulate nuclear/cytoplasmic shuttling of FOXO1: characterization of phosphorylation- and 14-3-3-dependent and -independent mechanisms." in: The Biochemical journal, Vol. 378, Issue Pt 3, pp. 839-49, (2004) (PubMed).

Rena, Woods, Prescott, Peggie, Unterman, Williams, Cohen: "Two novel phosphorylation sites on FKHR that are critical for its nuclear exclusion." in: The EMBO journal, Vol. 21, Issue 9, pp. 2263-71, (2002) (PubMed).

Rena, Prescott, Guo, Cohen, Unterman: "Roles of the forkhead in rhabdomyosarcoma (FKHR) phosphorylation sites in regulating 14-3-3 binding, transactivation and nuclear targetting." in: The Biochemical journal, Vol. 354, Issue Pt 3, pp. 605-12, (2001) (PubMed).

Guo, Rena, Cichy, He, Cohen, Unterman: "Phosphorylation of serine 256 by protein kinase B disrupts transactivation by FKHR and mediates effects of insulin on insulin-like growth factor-binding protein-1 promoter activity through a conserved insulin response sequence." in: The Journal of biological chemistry, Vol. 274, Issue 24, pp. 17184-92, (1999) (PubMed).

Target Details for FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Alternative Name: FKHR (FOXO1 Products)

Synonyms: FKH1 antibody, FKHR antibody, FOXO1A antibody, FoxO1a.1 antibody, foxo1 antibody, zgc:153388 antibody, Fkhr antibody, Foxo1a antibody, AI876417 antibody, Afxh antibody, Fkhr1 antibody, fkh1 antibody, fkhr antibody, foxo1a antibody, xFoxO1 antibody, FoxO1A antibody, foxo1a.2 antibody, forkhead box O1 antibody, forkhead box O1 a antibody, forkhead box O1 L homeolog antibody, forkhead box O1 b antibody, FOXO1 antibody, foxo1a antibody, Foxo1 antibody, foxo1 antibody, foxo1.L antibody, foxo1b antibody

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling