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Vimentin antibody (VIM)

Details for Product anti-VIM Antibody No. ABIN967252, Supplier: Log in to see
Antigen
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
Alternatives
anti-Human Vimentin antibody for Immunohistochemistry (Frozen Sections)
Reactivity
Human
572
166
148
72
53
41
27
24
15
10
9
9
8
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Host
Mouse
302
264
30
12
11
6
5
Clonality (Clone)
Monoclonal ()
Conjugate
This Vimentin antibody is un-conjugated
23
21
12
9
8
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
470
237
178
141
124
82
77
52
46
25
15
11
8
8
3
3
3
3
1
1
1
Supplier
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Immunogen Purified recombinant fragment of Vimentin expressed in E. Coli.
Clone 9E7E7-5G3F10-5G3F10E5
Isotype IgG1
Purification Ascitic fluid
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognising cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
Synonyms: FLJ36605, VIM
Gene ID 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis
Application Notes Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Vanzi: "Freedom at home: state constitutions and Medicaid funding for abortions." in: New Mexico law review, Vol. 26, Issue 3, pp. 433-54, 2005 (PubMed).

Background publications Essa, el Tatawi, Hamdi et al.: "Vimentin expression in different types of breast carcinoma immunohistochemical study." in: Journal of the Egyptian Society of Parasitology, Vol. 26, Issue 2, pp. 433-42, 1996 (PubMed).

Seshadri, Raymond, Leong et al.: "Vimentin expression is not associated with poor prognosis in breast cancer." in: International journal of cancer. Journal international du cancer, Vol. 67, Issue 3, pp. 353-6, 1996 (PubMed).

Chu, Seftor, Romer et al.: "Experimental coexpression of vimentin and keratin intermediate filaments in human melanoma cells augments motility." in: The American journal of pathology, Vol. 148, Issue 1, pp. 63-9, 1996 (PubMed).