CD72 B and C Alloantigens antibody

Catalog No. ABIN967281

This anti- antibody is a Rat Monoclonal antibody detecting in FACS, IP and WB. Suitable for Mouse. This Primary Antibody has been cited in 7+ publications.

Quick Overview for CD72 B and C Alloantigens antibody (ABIN967281)

Target: CD72 B and C Alloantigens

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)

Clone: JY-93

Product Details

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: BALB/c spleen cells

Isotype: IgG1 kappa

Application Details

Application Notes: Mouse Fc Block™ should not be used when staining with JY/93 mAb. This antyibody has been tested by Western Blot during development on 2PK-3 cell lysate and it recognizes a 45kDa band. However, it is not tested each lot by Western Blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References

Adachi, Wakabayashi, Nakayama, Yakura, Tsubata: "CD72 negatively regulates signaling through the antigen receptor of B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 3, pp. 1223-9, (2000) (PubMed).

Pan, Baumgarth, Parnes: "CD72-deficient mice reveal nonredundant roles of CD72 in B cell development and activation." in: Immunity, Vol. 11, Issue 4, pp. 495-506, (1999) (PubMed).

Bikah, Lynd, Aruffo, Ledbetter, Bondada: "A role for CD5 in cognate interactions between T cells and B cells, and identification of a novel ligand for CD5." in: International immunology, Vol. 10, Issue 8, pp. 1185-96, (1998) (PubMed).

Venkataraman, Lu, Buhl, Chen, Cambier, Bondada: "CD72-mediated B cell activation involves recruitment of CD19 and activation of phosphatidylinositol 3-kinase." in: European journal of immunology, Vol. 28, Issue 10, pp. 3003-16, (1998) (PubMed).

Leong, Marley, Loh, Robinson, Garlepp: "The induction of immune responses to murine malignant mesothelioma by IL-2 gene transfer." in: Immunology and cell biology, Vol. 75, Issue 4, pp. 356-9, (1997) (PubMed).

Robinson, Landolfi, Schäfer, Parnes: "Biochemical identity of the mouse Ly-19.2 and Ly-32.2 alloantigens with the B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 9, pp. 4764-72, (1993) (PubMed).

Robinson, Ying, Miceli, Parnes: "Extensive polymorphism in the extracellular domain of the mouse B cell differentiation antigen Lyb-2/CD72." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 3, pp. 880-6, (1992) (PubMed).

Target Details

Target: CD72 B and C Alloantigens

Background: The JY/93 antibody reacts with Lyb-2.2 and Lyb-2.3 (CD72 b and c alloantigens, respectively). CD72 is a 45-kDa type-II membrane protein containing a C-type lectin-like domain, an ITIM and ITIM-like sequence in the cytoplasmic tail. Recently, CD72 has been shown to negatively regulate B-cell receptor signaling. Analysis of CD72-deficient mice supports these results and shows that CD72 is involved in B-cell development. CD72-stimulated B cells show a transient association of CD19 with CD72, as well as an increase in Tyr-phosphorylation of CD19. CD72 is reported to be a ligand for CD5,5 although this is controversial. The CD72 alloantigens Lyb-2.1 (originally identified as Ly-m19.2), Lyb-2.2 (originally identified as Ly-32.2), Lyb-2.3, and Lyb-2.4 are encoded by the Cd72[a], Cd72[b], Cd72[c] , and Cd72[d] alleles, respectively. Lyb-2.2 (CD72b) is expressed on B lymphocytes, a subset of peripheral T cells, activated thymocytes, and activated T lymphocytes in A, BALB/c, CBA/H, CBA/N, C3H/He, C57BL, NZB, NZW, PL, and 129 strains. Moreover, expression of CD72b on T cells is dominant in F1 hybrid mice, and its level of expression increases upon activation. Lyb-2.3 (CD72c) is expressed on B cells of AKR, CE, SJL, and other mouse strains. Although staining of a small percentage of splenic T cells has been detected in CD72c mice, northern blot analysis has shown no expression of CD72c in T lymphocytes of these mice. JY/93 mAb does not stain cells from CD72a mice (e.g., CBA/J, DBA/2, SWR), and it does not stain resting thymocytes, splenic CD11b-positive cells, or NK cells. Five serological specificities of CD72 alloantigens have been described and the nomenclature CD72.1, CD72.2, CD72.3, CD72.4, and CD72.5 proposed.
Synonyms: Lyb-2.2 and Lyb-2.3