Cyclin D1 (CCND1) (full length) antibody

Details for Product No. ABIN967539
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Antigen
Synonyms
ccnd1b, prad1, bcl1, d11s287e, u21b31, ccndbp1, ccnd1, ZHM, CCND1, BCL1, D11S287E, PRAD1, U21B31, AI327039, CycD1, Cyl-1, bcl-1, cD1, ccnd1a, cb161, cycd1, etID37810.7, fb52e01, fc45c08, fc83a12, id:i ... show more
ccnd1b, prad1, bcl1, d11s287e, u21b31, ccndbp1, ccnd1, ZHM, CCND1, BCL1, D11S287E, PRAD1, U21B31, AI327039, CycD1, Cyl-1, bcl-1, cD1, ccnd1a, cb161, cycd1, etID37810.7, fb52e01, fc45c08, fc83a12, id:ibd1198, wu:fb52e01, wu:fc45c08, wu:fc83a12 show less
Epitope
full length
(46), (22), (18), (13), (8), (6), (5), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(278), (109), (101), (32), (19), (10), (9), (4), (2), (2), (1), (1), (1), (1), (1)
Host
Mouse
(235), (76), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(10), (8), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
(261), (92), (81), (60), (58), (42), (37), (32), (20), (18), (14), (11), (7), (3), (3), (3), (2), (1)
Pubmed 4 references available
Quantity 100 μg
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Catalog No. ABIN967539
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Immunogen Human Full-length Cyclin D1 Recombinant Protein
Clone DCS-6
Isotype IgG2a
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin D1
Background Cyclins and cyclin-dependent kinases (Cdks) have been shown to be subunits of cell cycle dependent protein kinases that regulate key events during the progression of the cell cycle and are evolutionarily highly conserved. Specific substrates for Cdk/cyclin kinases include nuclear lamins, histones, oncogenes (c-src, c-abl, SV40 large-T), tumor suppressor genes (the retinoblastoma protein [Rb] and p53), nucleolin, RNA polymerase II and others. D-type cyclins are involved in regulating the passage of mammalian cells through G1. In SDS-PAGE, D-type cyclins migrate at the following molecular weights: cyclin D1 (36 kDa), cyclin D2 (35 kDa), and cyclin D3 [31 and 34 kDa (doublet)]. Rodent cyclin D1 homologues (Cyl1) have been reported to typically migrate as a 36 kDa doublet. The mouse anti-human cyclin D1 antibody (clone DCS-6) recognizes human cyclin D1 (36 kDa) and has been reported to crossreact with rat and mouse cyclin D1 homologues (Cyl1). It does not crossreact with human cyclins D2 and D3.
Molecular Weight 36 kDa
Research Area Cancer, Cell Cycle
Comment

Related Products: ABIN968585, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Cyclin D1 (CCND1) (full length) antibody Western blot analysis of Cyclin D1. MCF7 cell lysates (Human breast adenocarcinoma, ATCC HTB-22) was probed with the mouse anti-human Cyclin D1 antibody (clone DCS-6) at 1-2 µg/mL (Lane 1). Cyclin D1 is identified as a band of ~36 kDa.
anti-Cyclin D1 (CCND1) (full length) antibody (2) anti-Cyclin D1 (CCND1) (full length) antibody (Image 2)
Product cited in: de Boer, Schuuring, Dreef et al.: "Cyclin D1 protein analysis in the diagnosis of mantle cell lymphoma." in: Blood, Vol. 86, Issue 7, pp. 2715-23, 1995 (PubMed).

Lukas, Pagano, Staskova et al.: "Cyclin D1 protein oscillates and is essential for cell cycle progression in human tumour cell lines." in: Oncogene, Vol. 9, Issue 3, pp. 707-18, 1994 (PubMed).

Meyerson, Harlow: "Identification of G1 kinase activity for cdk6, a novel cyclin D partner." in: Molecular and cellular biology, Vol. 14, Issue 3, pp. 2077-86, 1994 (PubMed).

Darzynkiewicz, Gong, Juan et al.: "Cytometry of cyclin proteins." in: Cytometry, Vol. 25, Issue 1, pp. 1-13, 1997 (PubMed).

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