CBL antibody (pTyr774)

Catalog No. ABIN967577

The Mouse Monoclonal anti-CBL antibody has been validated for WB and IHC (f). It is suitable to detect CBL in samples from Human. There are 2+ publications available.

Quick Overview for CBL antibody (pTyr774) (ABIN967577)

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CBL antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 29-c

Product Details anti-CBL Antibody

Binding Specificity: pTyr774

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human c-Cbl Peptide

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389, ABIN968537, ABIN968656

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-CBL antibody (ABIN967577)

Thien, Langdon: "Cbl: many adaptations to regulate protein tyrosine kinases." in: Nature reviews. Molecular cell biology, Vol. 2, Issue 4, pp. 294-307, (2001) (PubMed).

Tsygankov, Teckchandani, Feshchenko, Swaminathan: "Beyond the RING: CBL proteins as multivalent adapters." in: Oncogene, Vol. 20, Issue 44, pp. 6382-402, (2001) (PubMed).

Target Details for CBL

Target: CBL (Cas-Br-M (Murine) Ecotropic Retroviral Transforming Sequence (CBL))

Alternative Name: c-Cbl

Background: Cbl (Casitas B-lineage lymphoma) was identified in the genome of a transforming retrovirus from a mouse pre-B lymphoma. The cellular gene product c-Cbl is one of numerous Cbl-related proteins found in vertebrate and invertebrate organisms. It is an 120-kDa adapter protein that contains multiple functional domains, including a RING finger motif, a tyrosine kinase-binding (TKB) domain, and a proline-rich region. The TKB domain directly interacts with specific auto-phosphorylation sites in activated protein-tyrosine kinases (PTK). Through the RING finger motif, c-Cbl recruits and activates an E2 ubiquitin-conjugating enzyme, thus targeting the activated PTK for protein degradation. The proline-rich region contains SH3 domain-binding and 14-3-3 protein-binding motifs. c-Cbl is also phosphorylated at tyrosines 700, 731, and 774 (Y774) by Syk- and Src-family kinases after the stimulation of some integrins and a wide variety of receptors for antigens, immunoglobulins, growth factors, cytokines, and hormones. In turn, the phosphorylated Y774 site interacts with the SH2 domain of the CRK adapter protein. The c-Cbl adapter protein is expressed in the cytoplasm in all tissues, with especially high levels of expression in hematopoietic cells. Through its many functional sites, c-Cbl plays key roles in the positive and negative regulation of vital cell functions, including T Cell Receptor-mediated cellular immune responses. The 29/c-Cbl monoclonal antibody recognizes the Y774-phosphorylated form of human c-Cbl.

Pathways: TCR Signaling, Interferon-gamma Pathway, EGFR Signaling Pathway, EGFR Downregulation, VEGFR1 Specific Signals