Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (pTyr128) antibody

Antigen: Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)

Synonyms: SLP76, SLP-76

Binding Site: pTyr128

Clonality: Monoclonal (J141-668-36-58)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN967597

Additional Information

Alternative name: SLP-76

Immunogen: Phosphorylated Human SLP-76

Format: Liquid

Isotype: IgG1, kappa

Clone: J141-668-36-58

Description: SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR)-mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The phosphorylated tyrosine 128 (Y128) brings the Rho family guanine nucleotide exchange factor Vav1 and the Nck adapter protein, which binds to p21-activated kinase (PAK1) and Wiskott-Aldrich syndrome protein (WASP), into the activation complex.
Vav1, PAK1, and WASP may mediate TCR-stimulated actin cytoskeletal rearrangement. The J141-688.36.58 monoclonal antibody recognizes the phosphorylated Y128 of activated SLP-76.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 76 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of SLP-76 (pY128) in human T leukemia. Lysates from control and anti-CD3-activated Jurkat cells were probed with purified mouse anti-SLP-76 (pY128) monoclonal antibody at concentrations of 0.03125 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 myg/ml (lanes 3 and 6). SLP-76 (pY128) is identified as a band of 76 kDa in the treated cells.

Publications

Product: Fang, Motto, Ross et al.: "Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 3769-73, 1996 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, 2004 (PubMed).