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Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (pTyr113) antibody

Details for Product No. ABIN967606
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Antigen
Synonyms SLP76, SLP-76, AI323664, BB161688, twm, Slp76
Epitope
pTyr113
(14), (12), (12), (8), (6), (6), (3), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(105), (39), (32), (18), (14), (14)
Host
Mouse
(76), (21), (9), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application
Western Blotting (WB)
(78), (38), (30), (24), (20), (9), (2), (2), (2), (2), (1), (1)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967606
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Immunogen Phosphorylated Human SLP-76 Peptide
Clone J80-373
Isotype IgG1, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name SLP-76
Background SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR)-mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The phosphorylated tyrosine 113 (Y113) brings into the signaling complex the Rho-family guanine-nucleotide exchange factor Vav1, which is involved in the formation of a multimolecular assembly that participates in TCR-stimulated actin cytoskeletalrearrangement.
The J80-373 monoclonal antibody recognizes the phosphorylated Y113 of activated SLP-76.
Molecular Weight 76 kDa
Research Area Signaling
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (pTyr113) antibody Western blot analysis of SLP-76 (pY113) in human T leukemia. Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Jurkat cells were probed with purified mouse anti-SLP-76 (pY113) monoclonal antibody at concentrations of 0.0039 (lanes 1 and 4), 0.0019 (lanes 2 and 5), and 0.0010 myg/ml (lanes 3 and 6). SLP-76 (pY113) is identified as a band of 76 kDa in the treated cells.
Product cited in: Fang, Motto, Ross et al.: "Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 3769-73, 1996 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, 2004 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN967606
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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