Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2) (pTyr113) antibody

Antigen: Lymphocyte Cytosolic Protein 2 (SH2 Domain Containing Leukocyte Protein of 76kDa) (LCP2)

Synonyms: SLP76, SLP-76

Binding Site: pTyr113

Clonality: Monoclonal (J80-373)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN967606

Additional Information

Alternative name: SLP-76

Immunogen: Phosphorylated Human SLP-76 Peptide

Format: Liquid

Isotype: IgG1, kappa

Clone: J80-373

Description: SLP-76 (SH2 domain-containing Leukocyte Protein of 76 kDa) is a tyrosine phosphoprotein that is involved in the T cell receptor (TCR)-mediated intracellular signaling pathway. It may be involved in the signaling pathways of other peripheral blood leukocytes, thymic/splenic cells, and in human T, B, and monocytic cell lines. SLP-76 consists of several motifs that signify its importance in protein-protein interactions involved in intracellular signaling pathways, such as the SH2 domain in the C-terminus, the three amino-terminus 17-amino acid repeats with conserved tyrosine and acidic residues (DYE(S/P)P), and a proline rich region. SLP-76 has been shown to associate with Gads, Grb2, PLCg1, SLAP-130, and Vav, all of which are part of the signaling cascade in T lymphocytes. An early event in the T cell activation pathway is the phosphorylation, by the Syk-family kinase ZAP-70, of SLP-76 at the three conserved tyrosine motifs, which then mediate interactions with downstream effectors. The phosphorylated tyrosine 113 (Y113) brings into the signaling complex the Rho-family guanine-nucleotide exchange factor Vav1, which is involved in the formation of a multimolecular assembly that participates in TCR-stimulated actin cytoskeletalrearrangement.
The J80-373 monoclonal antibody recognizes the phosphorylated Y113 of activated SLP-76.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 76 kDa

Comments:

Related Products: ABIN967389

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Signaling

Restrictions: For Research Use only

Images

Western blot analysis of SLP-76 (pY113) in human T leukemia. Lysates from control (lanes 1-3) and hydrogen peroxide-activated (lanes 4-6) Jurkat cells were probed with purified mouse anti-SLP-76 (pY113) monoclonal antibody at concentrations of 0.0039 (lanes 1 and 4), 0.0019 (lanes 2 and 5), and 0.0010 myg/ml (lanes 3 and 6). SLP-76 (pY113) is identified as a band of 76 kDa in the treated cells.

Publications

Product: Fang, Motto, Ross et al.: "Tyrosines 113, 128, and 145 of SLP-76 are required for optimal augmentation of NFAT promoter activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 3769-73, 1996 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, 2004 (PubMed).