TANK-Binding Kinase 1 (TBK1) (pSer172) antibody

Details for Product No. ABIN967614
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Antigen
Synonyms nak, t2k, TBK1, wu:fk70c05, zgc:136548, NAK, T2K, 1200008B05Rik, AI462036, AW048562
Epitope
pSer172
(19), (15), (14), (13), (7), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(81), (46), (26), (15), (14), (12), (2)
Host
Mouse
(85), (10), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB)
(68), (41), (20), (15), (14), (10), (7), (6), (3), (2), (1), (1)
Pubmed 2 references available
Quantity 0.1 mg
Options
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Catalog No. ABIN967614
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Immunogen Phosphorylated Human TBK1
Clone J133-1171
Isotype IgG2b, kappa
Cross-Reactivity (Details) Predicted: Mouse
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name TBK1
Background NF-kB is a ubiquitously expressed transcription factor that regulates many cytokine and Ig genes. It is involved in immune, inflammatory, viral, and acute phase responses. In most cells, NF-kB is sequestered in an inactive cytoplasmic form via interactions with the inhibitory proteins IkBalpha, IkBbeta, and IkBepsilon. Stimulation induces the release, activation, and nuclear translocation of NF-kB. Release of NF-kB results from the phosphorylation and proteolytic degradation of the IkB proteins. Two cytokine-inducible IkappaB kinases (IKKa and IKKb) phosphorylate and target the IkB proteins for degradation via the ubiquitin pathway. IKKg/NEMO, a third member of the IKK complex, functions as a regulatory subunit and interacts directly with IKKb. TBK1 (TANK-binding kinase 1, also known as T2K or NAK), a protein of 729 amino acids, is another member of the IKK family of kinases regulating NF-kB downstream of the tumor necrosis factor and Toll-like receptor pathways. TBK1 forms a complex with the adaptor proteins TANK (TRAF-associated NF-kB activator) and TRAF2 (TNF-receptor-associated factor 2), and this oligomer is required for activation and phosphorylation of TBK1 at serine 172 (S172).
The J133-1171 monoclonal antibody recognizes the phosphorylated S172 of human TBK1.
Synonyms: T2K, NAK
Molecular Weight 80 kDa
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-TANK-Binding Kinase 1 (TBK1) (pSer172) antibody Western blot analysis of TBK1 (pS172) fusion protein. Lysates from control 293 fetal kidney cell line (first panel) and transfected 293T/IRF-7/TBK-1 cells (second panel) were probed with purified mouse anti-TBK1 (pS172) monoclonal antibody at concentrations of 0.0312 (lanes 1 and 4), 0.0156 (lanes 2 and 5), and 0.0078 µg/ml (lanes 3 and 6). TBK1-GFP fusion protein is identified as a band of 115 kDa in the transfected cells, which can be completely removed by treatment with lambda protein phosphatase (data not shown). The molecular weight of native human TBK1 protein has been reported to be 80 kDa.
Product cited in: Kishore, Huynh, Mathialagan et al.: "IKK-i and TBK-1 are enzymatically distinct from the homologous enzyme IKK-2: comparative analysis of recombinant human IKK-i, TBK-1, and IKK-2." in: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13840-7, 2002 (PubMed).

Viatour, Merville, Bours et al.: "Phosphorylation of NF-kappaB and IkappaB proteins: implications in cancer and inflammation." in: Trends in biochemical sciences, Vol. 30, Issue 1, pp. 43-52, 2005 (PubMed).

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