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TBK1 antibody (AA 514-527)

Verified TBK1 Reactivity: Human WB, ELISA, IF, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN1590112
  • Target See all TBK1 Antibodies
    TBK1 (TANK-Binding Kinase 1 (TBK1))
    Binding Specificity
    • 9
    • 8
    • 8
    • 7
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 514-527
    Reactivity
    • 70
    • 49
    • 28
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 74
    • 11
    Goat
    Clonality
    • 70
    • 15
    Polyclonal
    Conjugate
    • 45
    • 8
    • 8
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This TBK1 antibody is un-conjugated
    Application
    • 58
    • 41
    • 25
    • 11
    • 8
    • 6
    • 4
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
    Purpose
    TBK1 (aa514-527)
    Sequence
    TIETSLQDID SRLS
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Isotype
    IgG
    Top Product
    Discover our top product TBK1 Primary Antibody
  • Application Notes

    Western Blot: Approx 80 kDa band observed in lysates of cell line LNCaP and A431 (calculated MW of 83.6 kDa according to NP_037386.1). Recommended concentration: 0.5-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and in the cytoplasm and ER of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    TBK1 (TANK-Binding Kinase 1 (TBK1))
    Alternative Name
    TBK1 (TBK1 Products)
    Synonyms
    nak antibody, t2k antibody, TBK1 antibody, wu:fk70c05 antibody, zgc:136548 antibody, NAK antibody, T2K antibody, 1200008B05Rik antibody, AI462036 antibody, AW048562 antibody, TANK binding kinase 1 L homeolog antibody, TANK binding kinase 1 antibody, TANK-binding kinase 1 antibody, tbk1.L antibody, TBK1 antibody, tbk1 antibody, Tbk1 antibody
    Background
    TBK1, TANK-binding kinase 1, FLJ11330, NAK, T2K, NF-kB-activating kinase, NF-kappa-B-activating kinase, serine/threonine-protein kinase TBK1
    Gene ID
    29110
    NCBI Accession
    NP_037386
    Pathways
    TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
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